Alteration of beta-tubulin in Nicotiana plumbaginifolia confers resistance to amiprophos-methyl

YB Blume*, NM Strashnyuk, AP Smertenko, VG Solodushko, VA Sidorov, YY Gleba

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

A Nicotiana plumbaginifolia plant (apm5(r)) resistant to amiprophos-methyl (APM), a phosphoroamide herbicide, was isolated from protoplasts prepared from leaves of haploid plants. Genetic analysis revealed that the resistance is coded for by a dominant nuclear mutation and is associated with the increased stability of cortical microtubules. Two-dimensional polyacrylamide-gel electrophoresis, combined with immunoblotting using anti-tubulin monoclonal antibodies, showed that part of the beta-tubulin in the resistant plant possessed lower isoelectric points than the beta-tubulin of susceptible wild-type plants. These results provide evidence that the resistance to APM is associated with a mutation in a beta-tubulin gene. The APM-resistant line showed cross-resistance to trifluralin, a dinitroaniline herbicide, suggesting a common mechanism of resistance between these two classes of herbicides.

Original languageEnglish
Pages (from-to)464-472
Number of pages9
JournalTheoretical and Applied Genetics TAG
Volume97
Issue number3
Publication statusPublished - Aug 1998

Keywords

  • CHLAMYDOMONAS
  • DINITROANILINE-RESISTANT
  • mutation
  • MICROTUBULE POLYMERIZATION INVITRO
  • CELLS
  • beta-tubulin
  • PLANT TUBULIN
  • amiprophos-methyl
  • CROSS-RESISTANCE
  • microtubules
  • PROTEINS
  • resistance
  • HERBICIDE
  • ORYZALIN
  • INHIBITION

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