Altered pericyte-endothelial relations in the rat retina during aging: Implications for vessel stability

Suzanne Hughes, Tom Gardiner, Ping Hu, Louise Baxter, Emelia Rosinova, Tailoi Chan-Ling

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Mural cells (smooth muscle cells and pericytes) regulate blood flow and contribute to vessel stability. We examined whether mural cell changes accompany age-related alterations in the microvasculature of the central nervous system. The retinas of young adult and aged Wistar rats were subjected to immunohistofluorescence analysis of a-smooth muscle actin (SMA), caldesmon, calponin, desmin, and NG2 to identify mural cells. The vasculature was visualized by lectin histochemistry or perfusion of horse-radish peroxidase, and vessel walls were examined by electron microscopy. The early stage of aging was characterized by changes in peripheral retinal capillaries, including vessel broadening, thickening of the basement membrane, an altered length and orientation of desmin filaments in pericytes, a more widespread SMA distribution and changes in a subset of pre-arteriolar sphincters. In the later stages of aging, loss of capillary patency, aneurysms, distorted vessels, and foci of angiogenesis were apparent, especially in the peripheral deep vascular plexus. The capillary changes are consistent with impaired vascular autoregulation and may result in reduced pericyte-endothelial cell contact, destabilizing the capillaries and rendering them susceptible to angiogenic stimuli and endothelial cell loss as well as impairing the exchange of metabolites required for optimal neuronal function. This metabolic uncoupling leads to reactivation of
Original languageEnglish
Pages (from-to)1838-1847
Number of pages10
JournalNeurobiology of Aging
Volume27
Issue number12
Early online date18 Jan 2006
DOIs
Publication statusPublished - Dec 2006

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Pericytes
Retina
Desmin
Smooth Muscle
Blood Vessels
Actins
Endothelial Cells
Calmodulin-Binding Proteins
Raphanus
Microvessels
Lectins
Basement Membrane
Peroxidase
Horses
Smooth Muscle Myocytes
Aneurysm
Wistar Rats
Young Adult
Electron Microscopy
Homeostasis

Cite this

Hughes, Suzanne ; Gardiner, Tom ; Hu, Ping ; Baxter, Louise ; Rosinova, Emelia ; Chan-Ling, Tailoi. / Altered pericyte-endothelial relations in the rat retina during aging: Implications for vessel stability. In: Neurobiology of Aging. 2006 ; Vol. 27, No. 12. pp. 1838-1847.
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abstract = "Mural cells (smooth muscle cells and pericytes) regulate blood flow and contribute to vessel stability. We examined whether mural cell changes accompany age-related alterations in the microvasculature of the central nervous system. The retinas of young adult and aged Wistar rats were subjected to immunohistofluorescence analysis of a-smooth muscle actin (SMA), caldesmon, calponin, desmin, and NG2 to identify mural cells. The vasculature was visualized by lectin histochemistry or perfusion of horse-radish peroxidase, and vessel walls were examined by electron microscopy. The early stage of aging was characterized by changes in peripheral retinal capillaries, including vessel broadening, thickening of the basement membrane, an altered length and orientation of desmin filaments in pericytes, a more widespread SMA distribution and changes in a subset of pre-arteriolar sphincters. In the later stages of aging, loss of capillary patency, aneurysms, distorted vessels, and foci of angiogenesis were apparent, especially in the peripheral deep vascular plexus. The capillary changes are consistent with impaired vascular autoregulation and may result in reduced pericyte-endothelial cell contact, destabilizing the capillaries and rendering them susceptible to angiogenic stimuli and endothelial cell loss as well as impairing the exchange of metabolites required for optimal neuronal function. This metabolic uncoupling leads to reactivation of",
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Altered pericyte-endothelial relations in the rat retina during aging: Implications for vessel stability. / Hughes, Suzanne; Gardiner, Tom; Hu, Ping; Baxter, Louise; Rosinova, Emelia; Chan-Ling, Tailoi.

In: Neurobiology of Aging, Vol. 27, No. 12, 12.2006, p. 1838-1847.

Research output: Contribution to journalArticle

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