Amplification strategies and DNA biochips

Barbara Ruggeri, Laura Soverchia, Massimo Ubaldi, Roberto Ciccocioppo, Gary Hardiman

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Gene expression analysis has progressed rapidly over the past decade, from the historical analyses of individual genes to the sophisticated genome surveys routinely performed today. DNA biochips have facilitated this progression and helped accelerate target validation and drug discovery efforts by the pharmaceutical industry. The increasing use and acceptance of biochips to study genetic and cellular processes is clearly demonstrated by the large number of publications in recent years and the emergence of several robust commercial microarray platforms [1-3]. Affymetrix (Santa Clara, CA) pioneered this field and has been the market leader for many years, applying photolithographic technologies derived from the semiconductor industry to the fabrication of high-density 25-mer oligonucleotide microarrays. Alternative commercial platforms equally as robust have emerged, including array platforms from Agilent, Amersham, Illumina, and Applied Biosystems (see Chapter 1 of this book for further discussion of the technology platforms).

Original languageEnglish
Title of host publicationBiochips as Pathways to Drug Discovery
PublisherCRC Press
Pages253-260
Number of pages8
ISBN (Electronic)9781420015607
ISBN (Print)9781574444506
Publication statusPublished - 01 Jan 2006
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Ruggeri, B., Soverchia, L., Ubaldi, M., Ciccocioppo, R., & Hardiman, G. (2006). Amplification strategies and DNA biochips. In Biochips as Pathways to Drug Discovery (pp. 253-260). CRC Press.