Abstract
We describe an antibody-lectin sandwich assay for quantitation of glycoforms of proteins. The assay uses deglycosylated IgG antibody immobilized on a microtiter plate to capture the protein of interest from the sample. The particular glycoform is then identified by reaction with biotin-labeled lectin, which is measured using streptavidin/alkaline phosphatase. The assay can be adapted to quantitate any protein’s glycoforms by simply substituting the antibody and lectin with specific alternatives,
| Original language | English |
|---|---|
| Pages (from-to) | 203-206 |
| Number of pages | 4 |
| Journal | Molecular biotechnology |
| Volume | 12(2) |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Sept 1999 |
ASJC Scopus subject areas
- General Biochemistry,Genetics and Molecular Biology
- Biochemistry
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology