An antibody-lectin sandwich assay for quantifying protein glycoforms.

Fionnuala Lundy, G.B. Wisdom

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We describe an antibody-lectin sandwich assay for quantitation of glycoforms of proteins. The assay uses deglycosylated IgG antibody immobilized on a microtiter plate to capture the protein of interest from the sample. The particular glycoform is then identified by reaction with biotin-labeled lectin, which is measured using streptavidin/alkaline phosphatase. The assay can be adapted to quantitate any protein’s glycoforms by simply substituting the antibody and lectin with specific alternatives,
Original languageEnglish
Pages (from-to)203-206
Number of pages4
JournalMolecular biotechnology
Volume12(2)
Issue number2
DOIs
Publication statusPublished - Sep 1999

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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