An evolutionarily 'young' lysine residue in histone H3 attenuates transcriptional output in Saccharomyces cerevisiae

Edel M Hyland, Henrik Molina, Kunal Poorey, Chunfa Jie, Zhi Xie, Junbiao Dai, Jiang Qian, Stefan Bekiranov, David T Auble, Akhilesh Pandey, Jef D Boeke

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The DNA entry and exit points on the nucleosome core regulate the initial invasion of the nucleosome by factors requiring access to the underlying DNA. Here we describe in vivo consequences of eliminating a single protein-DNA interaction at this position through mutagenesis of histone H3 Lys 42 to alanine. This substitution has a dramatic effect on the Saccharomyces cerevisiae transcriptome in both the transcriptional output and landscape of mRNA species produced. We attribute this in part to decreased histone H3 occupancy at transcriptionally active loci, leading to enhanced elongation. Additionally we show that this lysine is methylated in vivo, and genetic studies of methyl-lysine mimics suggest that this modification may be crucial in attenuating gene expression. Interestingly, this site of methylation is unique to Ascomycota, suggesting a recent evolutionary innovation that highlights the evolvability of post-translational modifications of chromatin.

Original languageEnglish
Pages (from-to)1306-19
Number of pages14
JournalGenes & development
Volume25
Issue number12
DOIs
Publication statusPublished - 15 Jun 2011
Externally publishedYes

Keywords

  • Chromatin/chemistry
  • DNA Methylation
  • Evolution, Molecular
  • Gene Expression Profiling
  • Gene Expression Regulation, Fungal
  • Histones/chemistry
  • Lysine/chemistry
  • Models, Molecular
  • Mutation
  • Nuclear Proteins/metabolism
  • Peptide Elongation Factors/metabolism
  • Phenotype
  • Protein Structure, Tertiary
  • Saccharomyces cerevisiae/genetics
  • Saccharomyces cerevisiae Proteins/metabolism

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  • Cite this

    Hyland, E. M., Molina, H., Poorey, K., Jie, C., Xie, Z., Dai, J., Qian, J., Bekiranov, S., Auble, D. T., Pandey, A., & Boeke, J. D. (2011). An evolutionarily 'young' lysine residue in histone H3 attenuates transcriptional output in Saccharomyces cerevisiae. Genes & development, 25(12), 1306-19. https://doi.org/10.1101/gad.2050311