An in vitro investigation of endocrine disrupting effects of the mycotoxin alternariol

Caroline Frizzell, Doreen Ndossi, Shewit Kalayou, Gunnar S. Eriksen, Steven Verhaegen, Morten Sørlie, Christopher T. Elliott, Erik Ropstad, Lisa Connolly

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

Alternariol (AOH) is a mycotoxin commonly produced by Alternaria alternata on a wide range of foods. Few studies to date have been performed to evaluate the effects of AOH on endocrine activity. The present study makes use of in vitro mammalian cellular based assays and gene expression to investigate the ability of AOH to act as an endocrine disruptor by various modes of action. Reporter gene assays (RGAs), incorporating natural steroid hormone receptors for oestrogens, androgens, progestagens and glucocorticoids were used to identify endocrine disruption at the level of nuclear receptor transcriptional activity, and the H295R steroidogenesis assay was used to assess endocrine disruption at the level of gene expression and steroid hormone production. AOH exhibited a weak oestrogenic response when tested in the oestrogen responsive RGA and binding of progesterone to the progestagen receptor was shown to be synergistically increased in the presence of AOH. H295R cells when exposed to 0.1-1000ng/ml AOH, did not cause a significant change in testosterone and cortisol hormones but exposure to 1000ng/ml (3.87µM) AOH resulted in a significant increase in estradiol and progesterone production. In the gene expression study following exposure to 1000ng/ml (3.87µM) AOH, only one gene NR0B1 was down-regulated, whereas expression of mRNA for CYP1A1, MC2R, HSD3B2, CYP17, CYP21, CYP11B2 and CYP19 was up-regulated. Expression of the other genes investigated did not change significantly. In conclusion AOH is a weak oestrogenic mycotoxin that also has the ability to interfere with the steroidogenesis pathway.
Original languageEnglish
Pages (from-to)64-71
JournalToxicology and Applied Pharmacology
Volume271
Issue number1
Early online date09 May 2013
DOIs
Publication statusPublished - 15 Aug 2013

Bibliographical note

Copyright © 2013. Published by Elsevier Inc.

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Fingerprint Dive into the research topics of 'An in vitro investigation of endocrine disrupting effects of the mycotoxin alternariol'. Together they form a unique fingerprint.

Cite this