An Interlaboratory Comparison Study of Regulated and Emerging Mycotoxins Using Liquid Chromatography Mass Spectrometry: Challenges and Future Directions of Routine Multi-Mycotoxin Analysis including Emerging Mycotoxins

David Steiner, Armin Humpel, Eleonore Stamminger, Anna Schoeberl, Gerlinde Pachschwoell, Anita Sloboda, Christy Swoboda, Jolene Rigg, Dawei Zhang, Yahong Wang, Joshua Davis, Michael Sulyok, Rudolf Krska, Brian Quinn, Brett Greer, Christopher T Elliott, Zbynek Dzuman, Jana Hajslova, Andreas Gschaider, Carina FechnerLisa Forstner, Elisabeth Varga, Piotr Jedziniak, Katarzyna Pietruszka, Adrianna Rudawska, Alexandra Malachová

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Abstract

The present interlaboratory comparison study involved nine laboratories located throughout the world that tested for 24 regulated and non-regulated mycotoxins by applying their in-house LC-MS/MS multi-toxin method to 10 individual lots of 4 matrix commodities, including complex chicken and swine feed, soy and corn gluten. In total, more than 6000 data points were collected and analyzed statistically by calculating a consensus value in combination with a target standard deviation following a modified Horwitz equation. The performance of each participant was evaluated by a z-score assessment with a satisfying range of ±2, leading to an overall success rate of 70% for all tested compounds. Equal performance for both regulated and emerging mycotoxins indicates that participating routine laboratories have successfully expanded their analytical portfolio in view of potentially new regulations. In addition, the study design proved to be fit for the purpose of providing future certified reference materials, which surpass current analyte matrix combinations and exceed the typical scope of the regulatory framework.
Original languageEnglish
Article number405
JournalToxins
Volume14
Issue number6
DOIs
Publication statusPublished - 13 Jun 2022

Keywords

  • Chromatography, Liquid - methods
  • internal standard
  • Humans
  • Animals
  • method harmonization
  • Tandem Mass Spectrometry - methods
  • Chromatography, High Pressure Liquid - methods
  • z-score
  • complex feed
  • certified reference material
  • Glutens
  • Zea mays - chemistry
  • Swine
  • Mycotoxins - analysis

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