An invisible threat? Aspergillus positive cultures and co-infecting bacteria in airway samples

Dominic A Hughes, Mark Rosenthal, Leah Cuthbertson, Newara Ramadan, Imogen Felton, Nicholas J Simmonds, Michael R Loebinger, Henry Price, Darius Armstrong-James, J Stuart Elborn, William O Cookson, Miriam F Moffatt, Jane C Davies

Research output: Contribution to journalArticlepeer-review

Abstract

Background
Aspergillus fumigatus (Af) infection is associated with poor lung health in chronic suppurative lung diseases but often goes undetected. We hypothesised that inhibition of Af growth by Pseudomonas aeruginosa (Pa) increases the frequency of false-negative Af culture in co-infected people. Using a substantial group of cystic fibrosis (CF) airway samples, we assessed the relationship between Af and bacterial pathogens, additionally comparing fungal culture with next-generation sequencing.
Methods
Frequency of co-culture was assessed for 44,554 sputum/BAL cultures, from 1,367 CF patients between the years 2010–2020. In a subgroup, Internal Transcribed Spacer-2 (ITS2) fungal sequencing was used to determine sequencing-positive, culture-negative (S+/C-) rates.

Results
Pa+ samples were nearly 40% less likely (P<0.0001) than Pa- samples to culture Af, an effect that was also seen with some other Gram-negative isolates. This impact varied with Pa density and appeared to be moderated by Staphylococcus aureus co-infection. Sequencing identified Af-S+/C- for 40.1% of tested sputa. Samples with Pa had higher rates of Af-S+/C- (49.3%) than those without (35.7%; RR 1.38 [1.02–1.93], P<0.05). Af-S+/C- rate was not changed by other common bacterial infections. Pa did not affect the S+/C- rates of Candida, Exophiala or Scedosporium.

Conclusions
Pa/ Af co-positive cultures are less common than expected in CF. Our findings suggest an Af-positive culture is less likely in the presence of Pa. Interpretation of negative cultures should be cautious, particularly in Pa-positive samples, and a companion molecular diagnostic could be useful. Further work investigating mechanisms, alternative detection techniques and other chronic suppurative lung diseases is needed.
Original languageEnglish
JournalJournal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
Early online date21 Jul 2022
DOIs
Publication statusEarly online date - 21 Jul 2022

Keywords

  • Aspergillus
  • Co-infection
  • Chronic suppurative lung disease
  • Pseudomonas
  • Cystic fibrosis
  • Sequencing

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