An optimized method for the generation of AFLP markers in a stingless bee (Melipona quadrifasciata) reveals a high degree of intracolonial genetic polymorphism

G.R. Makert, Robert Paxton, K. Hartfelder

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study describes an optimized protocol for the generation of Amplified Fragment Length Polymorphism (AFLP) markers in a stingless bee. Essential modifications to standard protocols are a restriction enzyme digestion (EcoRI and Tru1I) in a two-step procedure, combined with a touchdown program in the selective PCR amplification step and product labelling by incorporation of alpha[P-33]dATP. In an analysis of 75 workers collected from three colonies of Melipona quadrifasciata we obtained 719 markers. Analysis of genetic variability revealed that on average 32% of the markers were polymorphic within a colony. Compared to the overall percentage of polymorphism (44% of the markers detected in our bee samples), the observed rates of within-colony polymorphism are remarkably high, considering that the workers of each colony were all of spring of a singly mated queen.
Original languageEnglish
Pages (from-to)687-698
Number of pages12
JournalAPIDOLOGIE
Volume37
Issue number6
DOIs
Publication statusPublished - Nov 2006

ASJC Scopus subject areas

  • Insect Science

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