Analysis of early hepatic stage schistosomula gene expression by subtractive expressed sequence tags library

Xinzhi Wang, Geoffrey N. Gobert, XinGang Feng, Zhiqiang Fu, Yamei Jin, Jinbiao Peng, JiaoJiao Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Schistosome parasites require a complex lifecycle requiring two hosts and aquatic phases of development. The schistosomula is a key phase of parasite development within the mammalian host, however relatively little is understood about the molecular processes underlying this stage. In this study 5723 subtractive expressed sequence tags (ESTs) were randomly selected from a 7 day hepatic schistosomula enriched library constructed using suppression subtractive hybridization method. Sequence analysis of these ESTs identified 1762 unique genes (contigs). Among them, 989 contigs were annotated with known genes, 311 contigs were homologous to established genes, 101 contigs were similar to established genes, 72 contigs were weakly similar to established genes and 289 sequences did not match any published sequences. Genes identified related to metabolism, cellular development, immune evasion and host-parasite interactions were identified as enriched in the hepatic schistosomula stage. The future identification of poorly annotated but stage-specific genes may potentially represent new drugs or vaccine targets, applicable for the future controlling of schistosomiasis.

Original languageEnglish
Pages (from-to)62-69
Number of pages8
JournalMolecular and Biochemical Parasitology
Volume166
Issue number1
DOIs
Publication statusPublished - 01 Jul 2009
Externally publishedYes

Keywords

  • Gene expression
  • Schistosoma japonicum
  • Schistosomula
  • Suppressed subtractive hybridization

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

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