Analysis of endocrine disruption in southern California coastal fish using an aquatic multispecies microarray

Michael E. Baker, Barbara Ruggeri, L. James Sprague, Colleen Eckhardt-Ludka, Jennifer Lapira, Ivan Wick, Laura Soverchia, Massimo Ubaldi, Alberta Maria Polzonetti-Magni, Doris Vidal-Dorsch, Steven Bay, Joseph R. Gully, Jesus A. Reyes, Kevin M. Kelley, Daniel Schlenk, Ellen C. Breen, Roman Šášik, Gary Hardiman*

*Corresponding author for this work

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Background: Endocrine disruptors include plasticizers, pesticides, detergents, and pharmaceuticals. Turbot and other flatfish are used to characterize the presence of chemicals in the marine environment. Unfortunately, there are relatively few genes of turbot and other flatfish in GenBank, which limits the use of molecular tools such as microarrays and quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) to study disruption of endocrine responses in sentinel fish captured by regulatory agencies. Objectives: We fabricated a multigene cross-species microarray as a diagnostic tool to screen the effects of environmental chemicals in fish, for which there is minimal genomic information. The array included genes that are involved in the actions of adrenal and sex steroids, thyroid hormone, and xenobiotic responses. This microarray will provide a sensitive tool for screening for the presence of chemicals with adverse effects on endocrine responses in coastal fish species. Methods: We used a custom multispecies microarray to study gene expression in wild hornyhead turbot (Pleuronichthys verticalis) collected from polluted and clean coastal waters and in laboratory male zebrafish (Danio rerio) after exposure to estradiol and 4-nonylphenol. We measured genespecific expression in turbot liver by qRT-PCR and correlated it to microarray data. Results: Microarray and qRT-PCR analyses of livers from turbot collected from polluted areas revealed altered gene expression profiles compared with those from nonaffected areas. Conclusions: The agreement between the array data and qRT-PCR analyses validates this multi species microarray. The microarray measurement of gene expression in zebrafish, which are phylogenetically distant from turbot, indicates that this multispecies microarray will be useful for measuring endocrine responses in other fish.

Original languageEnglish
Pages (from-to)223-230
Number of pages8
JournalEnvironmental Health Perspectives
Volume117
Issue number2
DOIs
Publication statusPublished - 27 Feb 2009
Externally publishedYes

Keywords

  • Danio rerio
  • Endocrine disruptors
  • Flatfish
  • Hornyhead turbot
  • Microarray
  • Multispecies array
  • Nonylphenol
  • Pleuronichthys verticalis
  • Xenobiotics
  • Xenoestrogens
  • Zebrafish

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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  • Cite this

    Baker, M. E., Ruggeri, B., Sprague, L. J., Eckhardt-Ludka, C., Lapira, J., Wick, I., Soverchia, L., Ubaldi, M., Polzonetti-Magni, A. M., Vidal-Dorsch, D., Bay, S., Gully, J. R., Reyes, J. A., Kelley, K. M., Schlenk, D., Breen, E. C., Šášik, R., & Hardiman, G. (2009). Analysis of endocrine disruption in southern California coastal fish using an aquatic multispecies microarray. Environmental Health Perspectives, 117(2), 223-230. https://doi.org/10.1289/ehp.11627