Angiotensin II pseudopeptides containing 1,3,5-trisubstituted benzene scaffolds with high AT2 receptor affinity

Jennie Georgsson, Christian Sköld, Bianca Plouffe, Gunnar Lindeberg, Milad Botros, Mats Larhed, Fred Nyberg, Nicole Gallo-Payet*, Adolf Gogoll, Anders Karlén, Anders Hallberg

*Corresponding author for this work

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Two 1,3,5-trisubstituted aromatic scaffolds intended to serve as γ-turn mimetics have been synthesized and incorporated in five pseudopeptide analogues of angiotensin II (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe), replacing Val-Tyr-Ile, Val-Tyr, or Tyr-Ile. All the tested compounds exhibited nanomolar affinity for the AT2 receptor with the best compound (3) having a Ki of 1.85 nM. Four pseudopeptides were AT2 selective, while one (5) also exhibited good affinity for the AT1 receptor (Ki = 30.3 nM). This pseudopeptide exerted full agonistic activity in an AT2 receptor induced neurite outgrowth assay but displayed no agonistic effect in an AT1 receptor functional assay. Molecular modeling, using the program DISCOtech, showed that the high-affinity ligands could interact similarly with the AT2 receptor as other ligands with high affinity for this receptor. A tentative agonist model is proposed for AT2 receptor activation by angiotensin II analogues. We conclude that the 1,3,5-trisubstituted benzene rings can be conveniently prepared and are suitable as γ-turn mimics.

Original languageEnglish
Pages (from-to)6620-6631
Number of pages12
JournalJournal of Medicinal Chemistry
Volume48
Issue number21
DOIs
Publication statusPublished - 20 Oct 2005
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

Fingerprint Dive into the research topics of 'Angiotensin II pseudopeptides containing 1,3,5-trisubstituted benzene scaffolds with high AT<sub>2</sub> receptor affinity'. Together they form a unique fingerprint.

  • Cite this

    Georgsson, J., Sköld, C., Plouffe, B., Lindeberg, G., Botros, M., Larhed, M., Nyberg, F., Gallo-Payet, N., Gogoll, A., Karlén, A., & Hallberg, A. (2005). Angiotensin II pseudopeptides containing 1,3,5-trisubstituted benzene scaffolds with high AT2 receptor affinity. Journal of Medicinal Chemistry, 48(21), 6620-6631. https://doi.org/10.1021/jm050280z