Antibody Responses to Fusobacterium nucleatum Proteins in Prediagnostic Blood Samples are not Associated with Risk of Developing Colorectal Cancer

Julia Butt; Mazda Jenab; Michael Pawlita; Kim Overvad; Anne Tjonneland; Anja Olsen; Marie-Christine Boutron-Ruault; Franck Carbonnel; Francesca Romana Mancini; Rudolf Kaaks; Tilman Kühn; Heiner Boeing; Antonia Trichopoulou; Anna Karakatsani; Domenico Palli; Valeria Maria Pala; Rosario Tumino; Carlotta Sacerdote; Salvatore Panico; Bas Bueno-de-Mesquita; Carla H van Gils; Roel C H Vermeulen; Elisabete Weiderpass; José Ramón Quirós; Eric Jeffrey Duell; Maria-Jose Sánchez; Miren Dorronsoro; José María Huerta; Eva Ardanaz; Bethany Van Guelpen; Sophia Harlid; Aurora Perez-Cornago; Marc J Gunter; Neil Murphy; Heinz Freisling; Dagfinn Aune; Tim Waterboer; David J Hughes

doi:10.1158/1055-9965.EPI-19-0313

Antibody Responses to Fusobacterium nucleatum Proteins in Prediagnostic Blood Samples are not Associated with Risk of Developing Colorectal Cancer

Julia Butt
, Mazda Jenab
, Michael Pawlita
, Kim Overvad
, Anne Tjonneland
, Anja Olsen
, Marie-Christine Boutron-Ruault
, Franck Carbonnel
, Francesca Romana Mancini
, Rudolf Kaaks
, Tilman Kühn
, Heiner Boeing
, Antonia Trichopoulou
, Anna Karakatsani
, Domenico Palli
, Valeria Maria Pala
, Rosario Tumino
, Carlotta Sacerdote
, Salvatore Panico
, Bas Bueno-de-Mesquita

Carla H van Gils, Roel C H Vermeulen, Elisabete Weiderpass, José Ramón Quirós, Eric Jeffrey Duell, Maria-Jose Sánchez, Miren Dorronsoro, José María Huerta, Eva Ardanaz, Bethany Van Guelpen, Sophia Harlid, Aurora Perez-Cornago, Marc J Gunter, Neil Murphy, Heinz Freisling, Dagfinn Aune, Tim Waterboer, David J Hughes

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

BACKGROUND: There is a lack of prospective data on the potential association of Fusobacterium nucleatum (F. nucleatum) and colorectal cancer risk. In this study, we assessed whether antibody responses to F. nucleatum are associated with colorectal cancer risk in prediagnostic serum samples in the European Prospective Investigation into Nutrition and Cancer (EPIC) cohort.

METHODS: We applied a multiplex serology assay to simultaneously measure antibody responses to 11 F. nucleatum antigens in prediagnostic serum samples from 485 colorectal cancer cases and 485 matched controls. Conditional logistic regression models were used to estimate odds ratios (ORs) and 95% confidence intervals (CI).

RESULTS: We observed neither a statistically significant colorectal cancer risk association for antibodies to individual F. nucleatum proteins nor for combined positivity to any of the 11 proteins (OR, 0.81; 95% CI, 0.62-1.06).

CONCLUSIONS: Antibody responses to F. nucleatum proteins in prediagnostic serum samples from a subset of colorectal cancer cases and matched controls within the EPIC study were not associated with colorectal cancer risk.

IMPACT: Our findings in prospectively ascertained serum samples contradict the existing literature on the association of F. nucleatum with colorectal cancer risk. Future prospective studies, specifically detecting F. nucleatum in stool or tissue biopsies, are needed to complement our findings.

Original language	English
Pages (from-to)	1552-1555
Number of pages	4
Journal	Cancer Epidemiology Biomarkers & Prevention
Volume	28
Issue number	9
DOIs	https://doi.org/10.1158/1055-9965.EPI-19-0313
Publication status	Published - 03 Sept 2019

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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Butt, J., Jenab, M., Pawlita, M., Overvad, K., Tjonneland, A., Olsen, A., Boutron-Ruault, M.-C., Carbonnel, F., Mancini, F. R., Kaaks, R., Kühn, T., Boeing, H., Trichopoulou, A., Karakatsani, A., Palli, D., Pala, V. M., Tumino, R., Sacerdote, C., Panico, S., ... Hughes, D. J. (2019). Antibody Responses to Fusobacterium nucleatum Proteins in Prediagnostic Blood Samples are not Associated with Risk of Developing Colorectal Cancer. Cancer Epidemiology Biomarkers & Prevention, 28(9), 1552-1555. https://doi.org/10.1158/1055-9965.EPI-19-0313

@article{add42c91592e4bc78340e71d41de9aa4,

title = "Antibody Responses to Fusobacterium nucleatum Proteins in Prediagnostic Blood Samples are not Associated with Risk of Developing Colorectal Cancer",

abstract = "BACKGROUND: There is a lack of prospective data on the potential association of Fusobacterium nucleatum (F. nucleatum) and colorectal cancer risk. In this study, we assessed whether antibody responses to F. nucleatum are associated with colorectal cancer risk in prediagnostic serum samples in the European Prospective Investigation into Nutrition and Cancer (EPIC) cohort.METHODS: We applied a multiplex serology assay to simultaneously measure antibody responses to 11 F. nucleatum antigens in prediagnostic serum samples from 485 colorectal cancer cases and 485 matched controls. Conditional logistic regression models were used to estimate odds ratios (ORs) and 95\% confidence intervals (CI).RESULTS: We observed neither a statistically significant colorectal cancer risk association for antibodies to individual F. nucleatum proteins nor for combined positivity to any of the 11 proteins (OR, 0.81; 95\% CI, 0.62-1.06).CONCLUSIONS: Antibody responses to F. nucleatum proteins in prediagnostic serum samples from a subset of colorectal cancer cases and matched controls within the EPIC study were not associated with colorectal cancer risk.IMPACT: Our findings in prospectively ascertained serum samples contradict the existing literature on the association of F. nucleatum with colorectal cancer risk. Future prospective studies, specifically detecting F. nucleatum in stool or tissue biopsies, are needed to complement our findings.",

author = "Julia Butt and Mazda Jenab and Michael Pawlita and Kim Overvad and Anne Tjonneland and Anja Olsen and Marie-Christine Boutron-Ruault and Franck Carbonnel and Mancini, \{Francesca Romana\} and Rudolf Kaaks and Tilman K{\"u}hn and Heiner Boeing and Antonia Trichopoulou and Anna Karakatsani and Domenico Palli and Pala, \{Valeria Maria\} and Rosario Tumino and Carlotta Sacerdote and Salvatore Panico and Bas Bueno-de-Mesquita and \{van Gils\}, \{Carla H\} and Vermeulen, \{Roel C H\} and Elisabete Weiderpass and Quir{\'o}s, \{Jos{\'e} Ram{\'o}n\} and Duell, \{Eric Jeffrey\} and Maria-Jose S{\'a}nchez and Miren Dorronsoro and Huerta, \{Jos{\'e} Mar{\'i}a\} and Eva Ardanaz and \{Van Guelpen\}, Bethany and Sophia Harlid and Aurora Perez-Cornago and Gunter, \{Marc J\} and Neil Murphy and Heinz Freisling and Dagfinn Aune and Tim Waterboer and Hughes, \{David J\}",

note = "{\textcopyright}2019 American Association for Cancer Research.",

year = "2019",

month = sep,

day = "3",

doi = "10.1158/1055-9965.EPI-19-0313",

language = "English",

volume = "28",

pages = "1552--1555",

journal = "Cancer Epidemiology Biomarkers \& Prevention",

issn = "1055-9965",

publisher = "American Association for Cancer Research Inc.",

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Butt, J, Jenab, M, Pawlita, M, Overvad, K, Tjonneland, A, Olsen, A, Boutron-Ruault, M-C, Carbonnel, F, Mancini, FR, Kaaks, R, Kühn, T, Boeing, H, Trichopoulou, A, Karakatsani, A, Palli, D, Pala, VM, Tumino, R, Sacerdote, C, Panico, S, Bueno-de-Mesquita, B, van Gils, CH, Vermeulen, RCH, Weiderpass, E, Quirós, JR, Duell, EJ, Sánchez, M-J, Dorronsoro, M, Huerta, JM, Ardanaz, E, Van Guelpen, B, Harlid, S, Perez-Cornago, A, Gunter, MJ, Murphy, N, Freisling, H, Aune, D, Waterboer, T & Hughes, DJ 2019, 'Antibody Responses to Fusobacterium nucleatum Proteins in Prediagnostic Blood Samples are not Associated with Risk of Developing Colorectal Cancer', Cancer Epidemiology Biomarkers & Prevention, vol. 28, no. 9, pp. 1552-1555. https://doi.org/10.1158/1055-9965.EPI-19-0313

TY - JOUR

T1 - Antibody Responses to Fusobacterium nucleatum Proteins in Prediagnostic Blood Samples are not Associated with Risk of Developing Colorectal Cancer

AU - Butt, Julia

AU - Jenab, Mazda

AU - Pawlita, Michael

AU - Overvad, Kim

AU - Tjonneland, Anne

AU - Olsen, Anja

AU - Boutron-Ruault, Marie-Christine

AU - Carbonnel, Franck

AU - Mancini, Francesca Romana

AU - Kaaks, Rudolf

AU - Kühn, Tilman

AU - Boeing, Heiner

AU - Trichopoulou, Antonia

AU - Karakatsani, Anna

AU - Palli, Domenico

AU - Pala, Valeria Maria

AU - Tumino, Rosario

AU - Sacerdote, Carlotta

AU - Panico, Salvatore

AU - Bueno-de-Mesquita, Bas

AU - van Gils, Carla H

AU - Vermeulen, Roel C H

AU - Weiderpass, Elisabete

AU - Quirós, José Ramón

AU - Duell, Eric Jeffrey

AU - Sánchez, Maria-Jose

AU - Dorronsoro, Miren

AU - Huerta, José María

AU - Ardanaz, Eva

AU - Van Guelpen, Bethany

AU - Harlid, Sophia

AU - Perez-Cornago, Aurora

AU - Gunter, Marc J

AU - Murphy, Neil

AU - Freisling, Heinz

AU - Aune, Dagfinn

AU - Waterboer, Tim

AU - Hughes, David J

PY - 2019/9/3

Y1 - 2019/9/3

N2 - BACKGROUND: There is a lack of prospective data on the potential association of Fusobacterium nucleatum (F. nucleatum) and colorectal cancer risk. In this study, we assessed whether antibody responses to F. nucleatum are associated with colorectal cancer risk in prediagnostic serum samples in the European Prospective Investigation into Nutrition and Cancer (EPIC) cohort.METHODS: We applied a multiplex serology assay to simultaneously measure antibody responses to 11 F. nucleatum antigens in prediagnostic serum samples from 485 colorectal cancer cases and 485 matched controls. Conditional logistic regression models were used to estimate odds ratios (ORs) and 95% confidence intervals (CI).RESULTS: We observed neither a statistically significant colorectal cancer risk association for antibodies to individual F. nucleatum proteins nor for combined positivity to any of the 11 proteins (OR, 0.81; 95% CI, 0.62-1.06).CONCLUSIONS: Antibody responses to F. nucleatum proteins in prediagnostic serum samples from a subset of colorectal cancer cases and matched controls within the EPIC study were not associated with colorectal cancer risk.IMPACT: Our findings in prospectively ascertained serum samples contradict the existing literature on the association of F. nucleatum with colorectal cancer risk. Future prospective studies, specifically detecting F. nucleatum in stool or tissue biopsies, are needed to complement our findings.

AB - BACKGROUND: There is a lack of prospective data on the potential association of Fusobacterium nucleatum (F. nucleatum) and colorectal cancer risk. In this study, we assessed whether antibody responses to F. nucleatum are associated with colorectal cancer risk in prediagnostic serum samples in the European Prospective Investigation into Nutrition and Cancer (EPIC) cohort.METHODS: We applied a multiplex serology assay to simultaneously measure antibody responses to 11 F. nucleatum antigens in prediagnostic serum samples from 485 colorectal cancer cases and 485 matched controls. Conditional logistic regression models were used to estimate odds ratios (ORs) and 95% confidence intervals (CI).RESULTS: We observed neither a statistically significant colorectal cancer risk association for antibodies to individual F. nucleatum proteins nor for combined positivity to any of the 11 proteins (OR, 0.81; 95% CI, 0.62-1.06).CONCLUSIONS: Antibody responses to F. nucleatum proteins in prediagnostic serum samples from a subset of colorectal cancer cases and matched controls within the EPIC study were not associated with colorectal cancer risk.IMPACT: Our findings in prospectively ascertained serum samples contradict the existing literature on the association of F. nucleatum with colorectal cancer risk. Future prospective studies, specifically detecting F. nucleatum in stool or tissue biopsies, are needed to complement our findings.

U2 - 10.1158/1055-9965.EPI-19-0313

DO - 10.1158/1055-9965.EPI-19-0313

M3 - Article

C2 - 31481495

SN - 1055-9965

VL - 28

SP - 1552

EP - 1555

JO - Cancer Epidemiology Biomarkers & Prevention

JF - Cancer Epidemiology Biomarkers & Prevention

IS - 9

ER -

Antibody Responses to Fusobacterium nucleatum Proteins in Prediagnostic Blood Samples are not Associated with Risk of Developing Colorectal Cancer

Abstract

Bibliographical note

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