Antimicrobial lipopeptide tridecaptin A1 selectively binds to Gram-negative lipid II

Stephen A Cochrane, Brandon Findlay, Alireza Bakhtiary, Jeella Z Acedo, Eva M Rodriguez-Lopez, Pascal Mercier, John C Vederas

Research output: Contribution to journalArticle

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Abstract

Tridecaptin A1 (TriA1) is a nonribosomal lipopeptide with selective antimicrobial activity against Gram-negative bacteria. Here we show that TriA1 exerts its bactericidal effect by binding to the bacterial cell-wall precursor lipid II on the inner membrane, disrupting the proton motive force. Biochemical and biophysical assays show that binding to the Gram-negative variant of lipid II is required for membrane disruption and that only the proton gradient is dispersed. The NMR solution structure of TriA1 in dodecylphosphocholine micelles with lipid II has been determined, and molecular modeling was used to provide a structural model of the TriA1-lipid II complex. These results suggest that TriA1 kills Gram-negative bacteria by a mechanism of action using a lipid-II-binding motif.

Original languageEnglish
Pages (from-to)11561-11566
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number41
DOIs
Publication statusPublished - 11 Oct 2016

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Lipopeptides
Gram-Negative Bacteria
Proton-Motive Force
Membranes
Structural Models
Micelles
Cell Wall
Protons
tridecaptins
muramyl-NAc-(pentapeptide)pyrophosphoryl-undecaprenol

Keywords

  • Journal Article

Cite this

Cochrane, Stephen A ; Findlay, Brandon ; Bakhtiary, Alireza ; Acedo, Jeella Z ; Rodriguez-Lopez, Eva M ; Mercier, Pascal ; Vederas, John C. / Antimicrobial lipopeptide tridecaptin A1 selectively binds to Gram-negative lipid II. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 41. pp. 11561-11566.
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Antimicrobial lipopeptide tridecaptin A1 selectively binds to Gram-negative lipid II. / Cochrane, Stephen A; Findlay, Brandon; Bakhtiary, Alireza; Acedo, Jeella Z; Rodriguez-Lopez, Eva M; Mercier, Pascal; Vederas, John C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 41, 11.10.2016, p. 11561-11566.

Research output: Contribution to journalArticle

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AB - Tridecaptin A1 (TriA1) is a nonribosomal lipopeptide with selective antimicrobial activity against Gram-negative bacteria. Here we show that TriA1 exerts its bactericidal effect by binding to the bacterial cell-wall precursor lipid II on the inner membrane, disrupting the proton motive force. Biochemical and biophysical assays show that binding to the Gram-negative variant of lipid II is required for membrane disruption and that only the proton gradient is dispersed. The NMR solution structure of TriA1 in dodecylphosphocholine micelles with lipid II has been determined, and molecular modeling was used to provide a structural model of the TriA1-lipid II complex. These results suggest that TriA1 kills Gram-negative bacteria by a mechanism of action using a lipid-II-binding motif.

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