Apoptosis: calling time on apoptosome activity

Colin Adrain, Seamus J Martin

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Apoptosis is a controlled form of cellular demolition, catalyzed by a family of cysteine proteases called caspases. In response to diverse proapoptotic stimuli, caspase-9 is recruited and activated within an oligomeric complex called the apoptosome. The apoptosome drives autocatalytic processing of caspase-9, triggering a proteolytic caspase cascade that results in the biochemical and morphological changes characteristic of cell death. It is unclear why caspase-9 undergoes autocatalytic processing following apoptosome recruitment, because interdomain processing is dispensable for caspase-9 activity. A study has shed light on this issue by demonstrating that caspase-9 processing within the apoptosome promotes its displacement from the complex, leading to inactivation of this protease. Thus, autoprocessing of caspase-9 within the apoptosome serves as a "molecular timer" that limits the proteolytic activity of this complex through displacement of bound caspase-9 molecules. This timer mechanism may enable cells to prevent low amounts of apoptosome activation from spiraling out of control unless sufficient numbers of apoptosomes are assembled within a particular time window, which would drive full-blown caspase activation and apoptosis.

Original languageEnglish
Pages (from-to)pe62
JournalScience Signaling
Volume2
Issue number91
DOIs
Publication statusPublished - 06 Oct 2009
Externally publishedYes

Keywords

  • Animals
  • Apoptosis/physiology
  • Apoptosomes/metabolism
  • Apoptotic Protease-Activating Factor 1/metabolism
  • Binding Sites
  • Caspase 9/metabolism
  • Catalysis
  • Humans
  • Models, Biological
  • Protein Binding
  • Time Factors

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