TY - JOUR
T1 - Apoptosis, toll-like, RIG-I-like and NOD-like receptors are pathways jointly induced by diverse respiratory bacterial and viral pathogens
AU - Martínez, Isidoro
AU - Oliveros, Juan C.
AU - Cuesta, Isabel
AU - de la Barrera, Jorge
AU - Ausina, Vicente
AU - Casals, Cristina
AU - de Lorenzo, Alba
AU - García, Ernesto
AU - García-Fojeda, Belén
AU - Garmendia, Junkal
AU - González-Nicolau, Mar
AU - Lacoma, Alicia
AU - Menéndez, Margarita
AU - Moranta, David
AU - Nieto, Amelia
AU - Ortín, Juan
AU - Pérez-González, Alicia
AU - Prat, Cristina
AU - Ramos-Sevillano, Elisa
AU - Regueiro, Verónica
AU - Rodriguez-Frandsen, Ariel
AU - Solís, Dolores
AU - Yuste, José
AU - Bengoechea, José A.
AU - Melero, José A.
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Lower respiratory tract infections are among the top five leading causes of human death. Fighting these infections is therefore a world health priority. Searching for induced alterations in host gene expression shared by several relevant respiratory pathogens represents an alternative to identify new targets for wide-range host-oriented therapeutics. With this aim, alveolar macrophages were independently infected with three unrelated bacterial (Streptococcus pneumoniae, Klebsiella pneumoniae, and Staphylococcus aureus) and two dissimilar viral (respiratory syncytial virus and influenza A virus) respiratory pathogens, all of them highly relevant for human health. Cells were also activated with bacterial lipopolysaccharide (LPS) as a prototypical pathogen-associated molecular pattern. Patterns of differentially expressed cellular genes shared by the indicated pathogens were searched by microarray analysis. Most of the commonly up-regulated host genes were related to the innate immune response and/or apoptosis, with Toll-like, RIG-I-like and NOD-like receptors among the top 10 signaling pathways with over-expressed genes. These results identify new potential broad-spectrum targets to fight the important human infections caused by the bacteria and viruses studied here.
AB - Lower respiratory tract infections are among the top five leading causes of human death. Fighting these infections is therefore a world health priority. Searching for induced alterations in host gene expression shared by several relevant respiratory pathogens represents an alternative to identify new targets for wide-range host-oriented therapeutics. With this aim, alveolar macrophages were independently infected with three unrelated bacterial (Streptococcus pneumoniae, Klebsiella pneumoniae, and Staphylococcus aureus) and two dissimilar viral (respiratory syncytial virus and influenza A virus) respiratory pathogens, all of them highly relevant for human health. Cells were also activated with bacterial lipopolysaccharide (LPS) as a prototypical pathogen-associated molecular pattern. Patterns of differentially expressed cellular genes shared by the indicated pathogens were searched by microarray analysis. Most of the commonly up-regulated host genes were related to the innate immune response and/or apoptosis, with Toll-like, RIG-I-like and NOD-like receptors among the top 10 signaling pathways with over-expressed genes. These results identify new potential broad-spectrum targets to fight the important human infections caused by the bacteria and viruses studied here.
KW - Bacterial infections
KW - Core of up-regulated genes
KW - Host response
KW - Respiratory pathogens
KW - Viral infections
UR - http://www.scopus.com/inward/record.url?scp=85018302835&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2017.00276
DO - 10.3389/fmicb.2017.00276
M3 - Article
AN - SCOPUS:85018302835
VL - 8
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
IS - MAR
M1 - 276
ER -