Applications of Mechanochemistry for the Synthesis of DNA on Ionic Liquid Supports

Christopher Johnston, Christopher Hardacre, Marie E. Migaud*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

The standard methodology for the synthesis of oligonucleotides is the solid‐phase method. Here, we report the first case of mechanochemistry being directly applied to synthesising a dinucleotide on a soluble support. While mechanochemistry eliminates the need to solubilise a supported oligomer in the solution phase for phosphoramidite coupling, the support still confers the benefit of being able to purify the product by precipitation or by simple liquid‐liquid extractions as needed. Furthermore, this study also explores the use of mechanochemistry in the initial functionalisation of the ionic liquid support with a nucleoside monomer.
Original languageEnglish
Pages (from-to)382-388
Number of pages7
JournalChemistry ‐ Methods
Volume1
Issue number8
Early online date05 May 2021
DOIs
Publication statusEarly online date - 05 May 2021

Keywords

  • Full Paper
  • Full Papers
  • DNA synthesis
  • mechanochemistry
  • ionic liquids
  • oligonucleotides
  • soluble supports

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