Approaches for the simultaneous detection of thiamphenicol, florfenicol and florfenicol amine using immunochemical techniques

Terence L. Fodey*, Suja E. George, Imelda M. Traynor, Philippe Delahaut, D. Glenn Kennedy, Christopher T. Elliott, Steven R.H. Crooks

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Thiamphenicol and florfenicol are antibacterial agents permitted for use as veterinary drugs in animals used for food production. However, as the EU has established maximum residue limits for both and the metabolite florfenicol amine, there is a requirement to monitor animal food products for their residues. In this study antisera were generated which can simultaneously detect thiamphenicol, florfenicol and florfenicol amine in an immunoassay. Details of the various coupling techniques employed to prepare immunogens and enzyme labels are provided and the antibodies produced have been assessed, in homologous and heterologous ELISA formats, with respect to sensitivity and specificity. It was found that while the antisera raised to thiamphenicol and florfenicol generally performed better in a heterologous set up, those raised to florfenicol amine were not only less affected by the assay format but also produced the most sensitive antibodies to all three target analytes. Antisera matched previous sensitivity (IC50<1ngmL-1) but had improved cross-reactivity (>100%) to thiamphenicol and florfenicol.

Original languageEnglish
Pages (from-to)30-37
Number of pages8
JournalJournal of Immunological Methods
Volume393
Issue number1-2
DOIs
Publication statusPublished - 10 May 2013

Keywords

  • Antibody
  • Florfenicol
  • Florfenicol amine
  • Hapten
  • Immunoassay
  • Thiamphenicol

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Approaches for the simultaneous detection of thiamphenicol, florfenicol and florfenicol amine using immunochemical techniques'. Together they form a unique fingerprint.

Cite this