Arginine-Rich Peptide-Based mRNA Nanocomplexes Efficiently Instigate Cytotoxic T Cell Immunity Dependent on the Amphipathic Organization of the Peptide.

VK Udhayakumar, A De Beuckelaer , Joanne McCaffrey, Cian McCrudden, JL Kirschman, Daryll Vanover, L Van Hoecke, K Roose, K Deswarte, BG De Geest, S Lienenklaus, PJ Santangelo, J Grooten, Helen McCarthy, S De Koker

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

To date, the mRNA delivery field has been heavily dominated by lipid-based systems. Reports on the use of nonlipid carriers for mRNA delivery in contrast are rare in the context of mRNA vaccination. This paper describes the potential of a cell-penetrating peptide containing the amphipathic RALA motif to deliver antigen-encoding mRNA to the immune system. RALA condenses mRNA into nanocomplexes that display acidic pH-dependent membrane disruptive properties. RALA mRNA nanocomplexes enable mRNA escape from endosomes and thereby allow expression of mRNA inside the dendritic cell cytosol. Strikingly, RALA mRNA nanocomplexes containing pseudouridine and 5-methylcytidine modified mRNA elicit potent cytolytic T cell responses against the antigenic mRNA cargo and show superior efficacy in doing so when compared to RALA mRNA nanocomplexes containing unmodified mRNA. RALA's unique sequence and structural organization are vital to act as mRNA vaccine vehicle, as arginine-rich peptide variants that lack the RALA motif show reduced mRNA complexation, impaired cellular uptake and lose the ability to transfect dendritic cells in vitro and to evoke T cell immunity in vivo.
Original languageEnglish
Article number1601412
JournalAdvanced Healthcare Materials
Volume6
Issue number13
Early online date24 Apr 2017
DOIs
Publication statusPublished - 06 Jul 2017

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