Arteriolar and venular patterning in retinas of mice selectively expressing VEGF isoforms.

I. Stalmans; Y.S. Ng; R. Rohan; M. Fruttiger; A. Bouche; A. Yuce; H. Fujisawa; B. Hermans; M. Shani; S. Jansen; D. Hicklin; D.J. Anderson; Tom Gardiner; H.P. Hammes; L. Moons; M. Dewerchin; D. Collen; P. Carmeliet; P.A. D'Amore

doi:10.1172/JCI200214362

Arteriolar and venular patterning in retinas of mice selectively expressing VEGF isoforms.

I. Stalmans, Y.S. Ng, R. Rohan, M. Fruttiger, A. Bouche, A. Yuce, H. Fujisawa, B. Hermans, M. Shani, S. Jansen, D. Hicklin, D.J. Anderson, Tom Gardiner, H.P. Hammes, L. Moons, M. Dewerchin, D. Collen, P. Carmeliet, P.A. D'Amore

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Abstract

The murine VEGF gene is alternatively transcribed to yield the VEGF120, VEGF164, and VEGF188 isoforms, which differ in their potential to bind to heparan sulfate and neuropilin-1 and to stimulate endothelial growth. Here, their role in retinal vascular development was studied in mice selectively expressing single isoforms. VEGF164/164 mice were normal, healthy, and had normal retinal angiogenesis. In contrast, VEGF120/120 mice exhibited severe defects in vascular outgrowth and patterning, whereas VEGF188/188 mice displayed normal venular outgrowth but impaired arterial development. It is noteworthy that neuropilin-1, a receptor for VEGF164, was predominantly expressed in retinal arterioles. These findings reveal distinct roles of the various VEGF isoforms in vascular patterning and arterial development in the retina.

Original language	English
Pages (from-to)	327-336
Number of pages	10
Journal	Journal of Clinical Investigation
Volume	109(3)
Issue number	3
DOIs	https://doi.org/10.1172/JCI200214362
Publication status	Published - Feb 2002

ASJC Scopus subject areas

General Medicine

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Stalmans, I., Ng, Y. S., Rohan, R., Fruttiger, M., Bouche, A., Yuce, A., Fujisawa, H., Hermans, B., Shani, M., Jansen, S., Hicklin, D., Anderson, D. J., Gardiner, T., Hammes, H. P., Moons, L., Dewerchin, M., Collen, D., Carmeliet, P., & D'Amore, P. A. (2002). Arteriolar and venular patterning in retinas of mice selectively expressing VEGF isoforms. Journal of Clinical Investigation, 109(3)(3), 327-336. https://doi.org/10.1172/JCI200214362

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title = "Arteriolar and venular patterning in retinas of mice selectively expressing VEGF isoforms.",

abstract = "The murine VEGF gene is alternatively transcribed to yield the VEGF120, VEGF164, and VEGF188 isoforms, which differ in their potential to bind to heparan sulfate and neuropilin-1 and to stimulate endothelial growth. Here, their role in retinal vascular development was studied in mice selectively expressing single isoforms. VEGF164/164 mice were normal, healthy, and had normal retinal angiogenesis. In contrast, VEGF120/120 mice exhibited severe defects in vascular outgrowth and patterning, whereas VEGF188/188 mice displayed normal venular outgrowth but impaired arterial development. It is noteworthy that neuropilin-1, a receptor for VEGF164, was predominantly expressed in retinal arterioles. These findings reveal distinct roles of the various VEGF isoforms in vascular patterning and arterial development in the retina.",

author = "I. Stalmans and Y.S. Ng and R. Rohan and M. Fruttiger and A. Bouche and A. Yuce and H. Fujisawa and B. Hermans and M. Shani and S. Jansen and D. Hicklin and D.J. Anderson and Tom Gardiner and H.P. Hammes and L. Moons and M. Dewerchin and D. Collen and P. Carmeliet and P.A. D'Amore",

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doi = "10.1172/JCI200214362",

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Stalmans, I, Ng, YS, Rohan, R, Fruttiger, M, Bouche, A, Yuce, A, Fujisawa, H, Hermans, B, Shani, M, Jansen, S, Hicklin, D, Anderson, DJ, Gardiner, T, Hammes, HP, Moons, L, Dewerchin, M, Collen, D, Carmeliet, P & D'Amore, PA 2002, 'Arteriolar and venular patterning in retinas of mice selectively expressing VEGF isoforms.', Journal of Clinical Investigation, vol. 109(3), no. 3, pp. 327-336. https://doi.org/10.1172/JCI200214362

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T1 - Arteriolar and venular patterning in retinas of mice selectively expressing VEGF isoforms.

AU - Stalmans, I.

AU - Ng, Y.S.

AU - Rohan, R.

AU - Fruttiger, M.

AU - Bouche, A.

AU - Yuce, A.

AU - Fujisawa, H.

AU - Hermans, B.

AU - Shani, M.

AU - Jansen, S.

AU - Hicklin, D.

AU - Anderson, D.J.

AU - Gardiner, Tom

AU - Hammes, H.P.

AU - Moons, L.

AU - Dewerchin, M.

AU - Collen, D.

AU - Carmeliet, P.

AU - D'Amore, P.A.

PY - 2002/2

Y1 - 2002/2

N2 - The murine VEGF gene is alternatively transcribed to yield the VEGF120, VEGF164, and VEGF188 isoforms, which differ in their potential to bind to heparan sulfate and neuropilin-1 and to stimulate endothelial growth. Here, their role in retinal vascular development was studied in mice selectively expressing single isoforms. VEGF164/164 mice were normal, healthy, and had normal retinal angiogenesis. In contrast, VEGF120/120 mice exhibited severe defects in vascular outgrowth and patterning, whereas VEGF188/188 mice displayed normal venular outgrowth but impaired arterial development. It is noteworthy that neuropilin-1, a receptor for VEGF164, was predominantly expressed in retinal arterioles. These findings reveal distinct roles of the various VEGF isoforms in vascular patterning and arterial development in the retina.

AB - The murine VEGF gene is alternatively transcribed to yield the VEGF120, VEGF164, and VEGF188 isoforms, which differ in their potential to bind to heparan sulfate and neuropilin-1 and to stimulate endothelial growth. Here, their role in retinal vascular development was studied in mice selectively expressing single isoforms. VEGF164/164 mice were normal, healthy, and had normal retinal angiogenesis. In contrast, VEGF120/120 mice exhibited severe defects in vascular outgrowth and patterning, whereas VEGF188/188 mice displayed normal venular outgrowth but impaired arterial development. It is noteworthy that neuropilin-1, a receptor for VEGF164, was predominantly expressed in retinal arterioles. These findings reveal distinct roles of the various VEGF isoforms in vascular patterning and arterial development in the retina.

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EP - 336

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

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Arteriolar and venular patterning in retinas of mice selectively expressing VEGF isoforms.

Abstract

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