Artificial receptors for the extraction of nucleoside metabolite 7-methylguanosine from aqueous media made by molecular imprinting

Aleksandra Krstulja, Coralie De Schutter, Patrick Favetta, Panagiotis Manesiotis, Luigi Agrofoglio

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A series of imprinted polymers targeting nucleoside metabolites, prepared using a template analogue approach, are presented. These were prepared following selection of the optimum functional monomer by solution association studies using 1H-NMR titrations whereby methacrylic acid was shown to be the strongest receptor with and affinity constant of 621 ± 51 L mol-1 vs. 110 ± 16 L mol-1 for acrylamide. The best performing polymers were prepared using methanol as porogenic co-solvent and although average binding site affinities were marginally reduced, 2.3×104 L mol-1 vs. 2.7×104 L mol-1 measured for a polymer prepared in acetonitrile, these polymers contained the highest number of binding sites, 5.27 μmol g-1¬¬ vs. 1.64 μmol g-1, while they also exhibited enhanced selectivity for methylated guanosine derivatives. When applied as sorbents in the extraction of nucleoside derivative cancer biomarkers from synthetic urine samples, significant sample clean-up and recoveries of up to 90% for 7-methylguanosine were achieved.
Original languageEnglish
Pages (from-to)12-18
Number of pages7
JournalJournal of Chromatography A
Volume1365
DOIs
Publication statusPublished - 24 Oct 2014

Keywords

  • Molecular imprinting
  • nucleosides
  • 7-methyl guanosine
  • bioanalysis
  • solid phase extraction (SPE)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Polymers and Plastics

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