Association between host immunological and pro-inflammatory mediators with survival in cystic fibrosis patients chronically colonised with Pseudomonas aeruginosa (PA)

Kelly Moffitt, Lorraine Martin, Andrew Jones, A.K. Webb, Michael Tunney, Madeleine Ennis, Joseph Elborn

Research output: Contribution to conferencePaper

Abstract

Introduction and Aims: Chronic PA pulmonary infection is associated with an increased host inflammatory response involving the release and activation of damaging inflammatory mediators. Quantification of these mediators may give an indication of lung damage and changes in clinical status and mortality outcome. The aim of this study was to determine whether serum IgG and IgA titre against PA in clinically stable, chronically colonised adult CF patients (n = 40) correlates with mortality and host inflammatory mediators.
Methods: Serum was assayed by ELISA for IgG and IgA against PA antigens. Sputum HNE, Cathepsin S and Cathepsin B were measured by spectrophotometric and fluorogenic assays with sputum IL-8 and TNFa, plasma IL-6 and urine TNFr1 measured by ELISA. Correlations were calculated between inflammatory mediators and antibody titre and results compared with a 10-year survival outcome. Results: A significant positive correlation was observed between IgG and IL-6 (p = 0.0005), TNFa (p = 0.0068), TNFr1 (p = 0.037), Cat S (p = 0.0106) and Cat B (p = 0.0006). No correlation was found between IgG and IL-8 or HNE (p = 0.6229, 0.3921 respectively). In addition, IL-6 levels positively correlated with mortality (p = 0.037). No correlations were found between IgA and any of the inflammatory mediators.
Conclusion: Increases in IgG titre and IL-6 levels were shown to be associated with 10 year mortality. Pro-inflammatory mediators correlated with IgG response, and suggest that IgG to PA is an immunological marker of chronic infection and survival.
Original languageEnglish
PagesS92
Publication statusPublished - 01 Jun 2012
EventEuropean Cystic Fibrosis Society Conference - Dublin, Ireland
Duration: 06 Jun 201209 Jun 2012

Conference

ConferenceEuropean Cystic Fibrosis Society Conference
CountryIreland
CityDublin
Period06/06/201209/06/2012

Fingerprint

Cystic Fibrosis
Pseudomonas aeruginosa
Immunoglobulin G
Survival
Interleukin-6
Immunoglobulin A
cathepsin S
Mortality
Sputum
Interleukin-8
Cats
Enzyme-Linked Immunosorbent Assay
Lung
Cathepsin B
Infection
Serum
Urine
Antigens
Antibodies

Bibliographical note

Published in the Journal of Cystic Fibrosis, Vol 11

Cite this

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title = "Association between host immunological and pro-inflammatory mediators with survival in cystic fibrosis patients chronically colonised with Pseudomonas aeruginosa (PA)",
abstract = "Introduction and Aims: Chronic PA pulmonary infection is associated with an increased host inflammatory response involving the release and activation of damaging inflammatory mediators. Quantification of these mediators may give an indication of lung damage and changes in clinical status and mortality outcome. The aim of this study was to determine whether serum IgG and IgA titre against PA in clinically stable, chronically colonised adult CF patients (n = 40) correlates with mortality and host inflammatory mediators.Methods: Serum was assayed by ELISA for IgG and IgA against PA antigens. Sputum HNE, Cathepsin S and Cathepsin B were measured by spectrophotometric and fluorogenic assays with sputum IL-8 and TNFa, plasma IL-6 and urine TNFr1 measured by ELISA. Correlations were calculated between inflammatory mediators and antibody titre and results compared with a 10-year survival outcome. Results: A significant positive correlation was observed between IgG and IL-6 (p = 0.0005), TNFa (p = 0.0068), TNFr1 (p = 0.037), Cat S (p = 0.0106) and Cat B (p = 0.0006). No correlation was found between IgG and IL-8 or HNE (p = 0.6229, 0.3921 respectively). In addition, IL-6 levels positively correlated with mortality (p = 0.037). No correlations were found between IgA and any of the inflammatory mediators.Conclusion: Increases in IgG titre and IL-6 levels were shown to be associated with 10 year mortality. Pro-inflammatory mediators correlated with IgG response, and suggest that IgG to PA is an immunological marker of chronic infection and survival.",
author = "Kelly Moffitt and Lorraine Martin and Andrew Jones and A.K. Webb and Michael Tunney and Madeleine Ennis and Joseph Elborn",
note = "Published in the Journal of Cystic Fibrosis, Vol 11; European Cystic Fibrosis Society Conference ; Conference date: 06-06-2012 Through 09-06-2012",
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Moffitt, K, Martin, L, Jones, A, Webb, AK, Tunney, M, Ennis, M & Elborn, J 2012, 'Association between host immunological and pro-inflammatory mediators with survival in cystic fibrosis patients chronically colonised with Pseudomonas aeruginosa (PA)', Paper presented at European Cystic Fibrosis Society Conference, Dublin, Ireland, 06/06/2012 - 09/06/2012 pp. S92.

Association between host immunological and pro-inflammatory mediators with survival in cystic fibrosis patients chronically colonised with Pseudomonas aeruginosa (PA). / Moffitt, Kelly; Martin, Lorraine; Jones, Andrew; Webb, A.K.; Tunney, Michael; Ennis, Madeleine; Elborn, Joseph.

2012. S92 Paper presented at European Cystic Fibrosis Society Conference, Dublin, Ireland.

Research output: Contribution to conferencePaper

TY - CONF

T1 - Association between host immunological and pro-inflammatory mediators with survival in cystic fibrosis patients chronically colonised with Pseudomonas aeruginosa (PA)

AU - Moffitt, Kelly

AU - Martin, Lorraine

AU - Jones, Andrew

AU - Webb, A.K.

AU - Tunney, Michael

AU - Ennis, Madeleine

AU - Elborn, Joseph

N1 - Published in the Journal of Cystic Fibrosis, Vol 11

PY - 2012/6/1

Y1 - 2012/6/1

N2 - Introduction and Aims: Chronic PA pulmonary infection is associated with an increased host inflammatory response involving the release and activation of damaging inflammatory mediators. Quantification of these mediators may give an indication of lung damage and changes in clinical status and mortality outcome. The aim of this study was to determine whether serum IgG and IgA titre against PA in clinically stable, chronically colonised adult CF patients (n = 40) correlates with mortality and host inflammatory mediators.Methods: Serum was assayed by ELISA for IgG and IgA against PA antigens. Sputum HNE, Cathepsin S and Cathepsin B were measured by spectrophotometric and fluorogenic assays with sputum IL-8 and TNFa, plasma IL-6 and urine TNFr1 measured by ELISA. Correlations were calculated between inflammatory mediators and antibody titre and results compared with a 10-year survival outcome. Results: A significant positive correlation was observed between IgG and IL-6 (p = 0.0005), TNFa (p = 0.0068), TNFr1 (p = 0.037), Cat S (p = 0.0106) and Cat B (p = 0.0006). No correlation was found between IgG and IL-8 or HNE (p = 0.6229, 0.3921 respectively). In addition, IL-6 levels positively correlated with mortality (p = 0.037). No correlations were found between IgA and any of the inflammatory mediators.Conclusion: Increases in IgG titre and IL-6 levels were shown to be associated with 10 year mortality. Pro-inflammatory mediators correlated with IgG response, and suggest that IgG to PA is an immunological marker of chronic infection and survival.

AB - Introduction and Aims: Chronic PA pulmonary infection is associated with an increased host inflammatory response involving the release and activation of damaging inflammatory mediators. Quantification of these mediators may give an indication of lung damage and changes in clinical status and mortality outcome. The aim of this study was to determine whether serum IgG and IgA titre against PA in clinically stable, chronically colonised adult CF patients (n = 40) correlates with mortality and host inflammatory mediators.Methods: Serum was assayed by ELISA for IgG and IgA against PA antigens. Sputum HNE, Cathepsin S and Cathepsin B were measured by spectrophotometric and fluorogenic assays with sputum IL-8 and TNFa, plasma IL-6 and urine TNFr1 measured by ELISA. Correlations were calculated between inflammatory mediators and antibody titre and results compared with a 10-year survival outcome. Results: A significant positive correlation was observed between IgG and IL-6 (p = 0.0005), TNFa (p = 0.0068), TNFr1 (p = 0.037), Cat S (p = 0.0106) and Cat B (p = 0.0006). No correlation was found between IgG and IL-8 or HNE (p = 0.6229, 0.3921 respectively). In addition, IL-6 levels positively correlated with mortality (p = 0.037). No correlations were found between IgA and any of the inflammatory mediators.Conclusion: Increases in IgG titre and IL-6 levels were shown to be associated with 10 year mortality. Pro-inflammatory mediators correlated with IgG response, and suggest that IgG to PA is an immunological marker of chronic infection and survival.

M3 - Paper

SP - S92

ER -