Atmospheric pressure non-thermal plasma exposure reduces Pseudomonas aeruginosa lipopolysaccharide toxicity in vitro and in vivo

Muna M. Barakat, Yahya H. Dallal Bashi, Louise Carson, William G. Graham, Brendan F. Gilmore, Padrig B. Flynn*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Lipopolysaccharide (LPS) is an endotoxin composed of a polysaccharide and lipid component. It is intrinsically responsible for the pathogenicity of Gram-negative bacteria and is involved in the development of bacterial sepsis. Atmospheric pressure non-thermal plasma is proposed as a potential new approach for the treatment of infected tissue such as chronic wounds, with both antibacterial and wound-healing activities extensively described. Using both the RAW264.7 murine macrophage cell line in vitro assays and the Galleria mellonella insect in vivo toxicity model, the effect non-thermal plasma exposure on LPS-mediated toxicity has been characterised. Short (60 s) non-thermal plasma exposures of Pseudomonas aeruginosa conditioned growth media, membrane lysates and purified P. aeruginosa LPS, resulted in a substantial detoxification and reduction of LPS-induced cytotoxicity in RAW264.7 murine macrophages. Non-thermal plasma exposure (60 s) of purified P. aeruginosa LPS led to a significant (p < 0.05) improvement in the G. mellonella health index (GHI) score, a measure of in vivo toxicity. These findings demonstrate the ability of short plasma exposures to significantly reduce LPS-induced cytotoxicity both in vitro and in vivo; attenuating the toxicity of this important virulence factor intrinsic to the pathogenicity of Gram-negative bacteria.

Original languageEnglish
Article number103679
Number of pages6
JournalMicrobial Pathogenesis
Volume136
Early online date19 Aug 2019
DOIs
Publication statusPublished - 01 Nov 2019

Keywords

  • Cytotoxicity
  • Galleria mellonella
  • LPS
  • Macrophage
  • Non-thermal plasma
  • Virulence

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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