Abstract
Aims
The main aim of this study was to determine the virucidal inactivation efficacy of an in-house-designed atmospheric pressure, nonthermal plasma jet operated at varying helium/oxygen feed gas concentrations against MS2 bacteriophage, widely employed as a convenient surrogate for human norovirus.
Methods and Results
The effect of variation of percentage oxygen concentration in the helium (He) carrier gas was studied and found to positively correlate with MS2 inactivation rate, indicating a role for reactive oxygen species (ROS) in viral inactivation. The inactivation rate constant increased with increasing oxygen concentrations up to 075% O-2. 3 log(10) (999%) reductions in MS2 viability were achieved after 3min of exposure to the plasma source operated in a helium/oxygen (9925%:075%) gas mixture, with >7 log(10) reduction after 9min exposure.
Conclusions
Atmospheric pressure, nonthermal plasmas may have utility in the rapid disinfection of virally contaminated surfaces for infection control applications.
Significance and Impact of StudyThe atmospheric pressure, nonthermal plasma jet employed in this study exhibits rapid virucidal activity against a norovirus surrogate virus, the MS2 bacteriophage, which is superior to previously published inactivation rates for chemical disinfectants.
Original language | English |
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Pages (from-to) | 1420-1426 |
Journal | Journal of Applied Microbiology |
Volume | 115 |
Issue number | 6 |
Early online date | 12 Sept 2013 |
DOIs | |
Publication status | Published - Dec 2013 |
Bibliographical note
This article is protected by copyright. All rights reserved.Keywords
- atmospheric pressure plasma
- disinfection
- MS2 bacteriophage
- nonthermal plasma
- norovirus
- plasma sterilization
- HUMAN NOROVIRUS
- MECHANISMS
- GASTROENTERITIS
- STERILIZATION
- DISINFECTANTS
- SURROGATES
- MODEL
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Biotechnology