Au-nanoarray by Nanosphere Lithography Sensors for Staphylococcus aureus 16S rRNA detection

Agnes Purwidyantri; Chao Sung Lai

doi:10.1109/NANO.2016.7751569

Au-nanoarray by Nanosphere Lithography Sensors for Staphylococcus aureus 16S rRNA detection

Agnes Purwidyantri
, Chao Sung Lai

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

17 Downloads (Pure)

Abstract

Nanopatterning of Au-nanoarray on an indium tin oxide (ITO) electrode using efficient and low-cost nanospheres lithography (NSL) is reported. Convective self-assembly of 500 nm polystyrene (PS) nanobeads was found to effectively provide a well-ordered monolayer template for Au deposition with the coverage of about 90% on ITO. A simple Au-nanoparticles (AuNPs) spin coating was performed. It is observed that AuNPs immobilized by spin-coating techniques tends to follow the contour of the spherical nanobeads instead of filling the gaps within them, therefore, a distinguished hexagonal ring of Au-nanoarray was generated after nanobeads etching. As used for electrochemical sensor of Staphyloccocus aureus 16S rRNA hybridization detection, this substrate resulted high effective surface area which subsequently gave an excellent limitation of detection (LOD) of 10 pM target DNA. Overall, we have demonstrated an integration of NSL and AuNPs spin-coating for a uniform and high troughput substrate fabrication for electrochemical biosensor development.

Original language	English
Title of host publication	2016 IEEE 16th International Conference on Nanotechnology (IEEE-NANO): Proceedings
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	401-404
Number of pages	4
DOIs	https://doi.org/10.1109/NANO.2016.7751569
Publication status	Published - 24 Nov 2016
Externally published	Yes
Event	Au-nanoarray by Nanosphere Lithography Sensors for Staphylococcus aureus 16S rRNA - Japan, Sendai, Japan Duration: 22 Aug 2016 → 25 Aug 2016 https://ieeexplore.ieee.org/xpl/conhome/7742133/proceeding

Conference

Conference	Au-nanoarray by Nanosphere Lithography Sensors for Staphylococcus aureus 16S rRNA
Country/Territory	Japan
City	Sendai
Period	22/08/2016 → 25/08/2016
Internet address	https://ieeexplore.ieee.org/xpl/conhome/7742133/proceeding

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10.1109/NANO.2016.7751569

Au-nanoarray by Nanosphere Lithography Sensors for Staphylococcus aureus 16S rRNA detection
© 2016 The Authors. This work is made available online in accordance with the publisher’s policies. Please refer to any applicable terms of use of the publisher.
Accepted author manuscript, 1.14 MBLicence: Unspecified

Cite this

@inproceedings{f6464e051e07481986eac10cf5c35ece,

title = "Au-nanoarray by Nanosphere Lithography Sensors for Staphylococcus aureus 16S rRNA detection",

abstract = "Nanopatterning of Au-nanoarray on an indium tin oxide (ITO) electrode using efficient and low-cost nanospheres lithography (NSL) is reported. Convective self-assembly of 500 nm polystyrene (PS) nanobeads was found to effectively provide a well-ordered monolayer template for Au deposition with the coverage of about 90\% on ITO. A simple Au-nanoparticles (AuNPs) spin coating was performed. It is observed that AuNPs immobilized by spin-coating techniques tends to follow the contour of the spherical nanobeads instead of filling the gaps within them, therefore, a distinguished hexagonal ring of Au-nanoarray was generated after nanobeads etching. As used for electrochemical sensor of Staphyloccocus aureus 16S rRNA hybridization detection, this substrate resulted high effective surface area which subsequently gave an excellent limitation of detection (LOD) of 10 pM target DNA. Overall, we have demonstrated an integration of NSL and AuNPs spin-coating for a uniform and high troughput substrate fabrication for electrochemical biosensor development. ",

author = "Agnes Purwidyantri and Lai, \{Chao Sung\}",

year = "2016",

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doi = "10.1109/NANO.2016.7751569",

language = "English",

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booktitle = "2016 IEEE 16th International Conference on Nanotechnology (IEEE-NANO): Proceedings",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

address = "United States",

note = "Au-nanoarray by Nanosphere Lithography Sensors for Staphylococcus aureus 16S rRNA ; Conference date: 22-08-2016 Through 25-08-2016",

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}

Purwidyantri, A & Lai, CS 2016, Au-nanoarray by Nanosphere Lithography Sensors for Staphylococcus aureus 16S rRNA detection. in 2016 IEEE 16th International Conference on Nanotechnology (IEEE-NANO): Proceedings. Institute of Electrical and Electronics Engineers Inc., pp. 401-404, Au-nanoarray by Nanosphere Lithography Sensors for Staphylococcus aureus 16S rRNA , Sendai, Japan, 22/08/2016. https://doi.org/10.1109/NANO.2016.7751569

Au-nanoarray by Nanosphere Lithography Sensors for Staphylococcus aureus 16S rRNA detection. / Purwidyantri, Agnes; Lai, Chao Sung.
2016 IEEE 16th International Conference on Nanotechnology (IEEE-NANO): Proceedings. Institute of Electrical and Electronics Engineers Inc., 2016. p. 401-404.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Au-nanoarray by Nanosphere Lithography Sensors for Staphylococcus aureus 16S rRNA detection

AU - Purwidyantri, Agnes

AU - Lai, Chao Sung

PY - 2016/11/24

Y1 - 2016/11/24

N2 - Nanopatterning of Au-nanoarray on an indium tin oxide (ITO) electrode using efficient and low-cost nanospheres lithography (NSL) is reported. Convective self-assembly of 500 nm polystyrene (PS) nanobeads was found to effectively provide a well-ordered monolayer template for Au deposition with the coverage of about 90% on ITO. A simple Au-nanoparticles (AuNPs) spin coating was performed. It is observed that AuNPs immobilized by spin-coating techniques tends to follow the contour of the spherical nanobeads instead of filling the gaps within them, therefore, a distinguished hexagonal ring of Au-nanoarray was generated after nanobeads etching. As used for electrochemical sensor of Staphyloccocus aureus 16S rRNA hybridization detection, this substrate resulted high effective surface area which subsequently gave an excellent limitation of detection (LOD) of 10 pM target DNA. Overall, we have demonstrated an integration of NSL and AuNPs spin-coating for a uniform and high troughput substrate fabrication for electrochemical biosensor development.

AB - Nanopatterning of Au-nanoarray on an indium tin oxide (ITO) electrode using efficient and low-cost nanospheres lithography (NSL) is reported. Convective self-assembly of 500 nm polystyrene (PS) nanobeads was found to effectively provide a well-ordered monolayer template for Au deposition with the coverage of about 90% on ITO. A simple Au-nanoparticles (AuNPs) spin coating was performed. It is observed that AuNPs immobilized by spin-coating techniques tends to follow the contour of the spherical nanobeads instead of filling the gaps within them, therefore, a distinguished hexagonal ring of Au-nanoarray was generated after nanobeads etching. As used for electrochemical sensor of Staphyloccocus aureus 16S rRNA hybridization detection, this substrate resulted high effective surface area which subsequently gave an excellent limitation of detection (LOD) of 10 pM target DNA. Overall, we have demonstrated an integration of NSL and AuNPs spin-coating for a uniform and high troughput substrate fabrication for electrochemical biosensor development.

U2 - 10.1109/NANO.2016.7751569

DO - 10.1109/NANO.2016.7751569

M3 - Conference contribution

SP - 401

EP - 404

BT - 2016 IEEE 16th International Conference on Nanotechnology (IEEE-NANO): Proceedings

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - Au-nanoarray by Nanosphere Lithography Sensors for Staphylococcus aureus 16S rRNA

Y2 - 22 August 2016 through 25 August 2016

ER -

Au-nanoarray by Nanosphere Lithography Sensors for Staphylococcus aureus 16S rRNA detection

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