Abstract
We describe a centrifugal microfluidic 'Lab-on-a-Disc' (LoaD) technology for DNA purification towards eventual integration into a Sample-to-Answer platform for detection of the pathogen Escherichia coli O157:H7 from food samples. For this application, we use a novel microfluidic architecture which combines 'event-triggered' dissolvable film (DF) valves with a reaction chamber gated by a centrifugo-pneumatic siphon valve (CPSV). This architecture permits comprehensive flow control by simple changes in the speed of the platform innate spindle motor. Even before method optimisation, characterisation by DNA fluorescence reveals an extraction efficiency of 58%, which is close to commercial spin columns.
Original language | English |
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Title of host publication | Proceedings of the 27th Micromechanics and Microsystems Europe Workshop, MME 2016 |
Publisher | IOP Publishing Ltd |
Number of pages | 7 |
DOIs | |
Publication status | Published - 11 Nov 2016 |
Externally published | Yes |
Event | 27th Micromechanics and Microsystems Europe Workshop 2016 - Cork, Ireland Duration: 28 Aug 2016 → 30 Aug 2016 |
Publication series
Name | Journal of Physics: Conference Series |
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Volume | 757 |
ISSN (Print) | 1742-6588 |
Workshop
Workshop | 27th Micromechanics and Microsystems Europe Workshop 2016 |
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Abbreviated title | MME 2016 |
Country/Territory | Ireland |
City | Cork |
Period | 28/08/2016 → 30/08/2016 |
ASJC Scopus subject areas
- General Physics and Astronomy
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Dive into the research topics of 'Automation of silica bead-based nucleic acid extraction on a centrifugal lab-on-a-disc platform'. Together they form a unique fingerprint.Student theses
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Synthesis, characterisation, and spectral metrology of plasmonic titanium nitride films and nanostructures
Lipinski, A. F. (Author), Bowman, R. (Supervisor) & Arredondo-Arechavala, M. (Supervisor), Jul 2024Student thesis: Doctoral Thesis › Doctor of Philosophy
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