Automation of silica bead-based nucleic acid extraction on a centrifugal lab-on-a-disc platform

David J. Kinahan*, Faith Mangwanya, Robert Garvey, Danielle W. Y. Chung, Artur Lipinski, Lourdes A.N. Julius, Damien King, Mehdi Mohammadi, Rohit Mishra, May Al-Ofi, Celina Miyazaki, Jens Ducrée*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

17 Citations (Scopus)
16 Downloads (Pure)

Abstract

We describe a centrifugal microfluidic 'Lab-on-a-Disc' (LoaD) technology for DNA purification towards eventual integration into a Sample-to-Answer platform for detection of the pathogen Escherichia coli O157:H7 from food samples. For this application, we use a novel microfluidic architecture which combines 'event-triggered' dissolvable film (DF) valves with a reaction chamber gated by a centrifugo-pneumatic siphon valve (CPSV). This architecture permits comprehensive flow control by simple changes in the speed of the platform innate spindle motor. Even before method optimisation, characterisation by DNA fluorescence reveals an extraction efficiency of 58%, which is close to commercial spin columns.

Original languageEnglish
Title of host publicationProceedings of the 27th Micromechanics and Microsystems Europe Workshop, MME 2016
PublisherIOP Publishing Ltd
Number of pages7
DOIs
Publication statusPublished - 11 Nov 2016
Externally publishedYes
Event27th Micromechanics and Microsystems Europe Workshop 2016 - Cork, Ireland
Duration: 28 Aug 201630 Aug 2016

Publication series

NameJournal of Physics: Conference Series
Volume757
ISSN (Print)1742-6588

Workshop

Workshop27th Micromechanics and Microsystems Europe Workshop 2016
Abbreviated titleMME 2016
Country/TerritoryIreland
CityCork
Period28/08/201630/08/2016

ASJC Scopus subject areas

  • General Physics and Astronomy

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