Avoiding pitfalls in proteomics sample preparation

Robert L.J. Graham, Anastasia Kalli, Geoffrey T. Smith, Michael J. Sweredoski, Sonja Hess*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The purpose of this chapter is to focus on areas that are often underestimated in their importance for optimized results in mass spectrometric analyses, namely sample preparation. While there is no doubt that the advancements in technology led us to where we are no-it is also clear that a deep understanding of appropriate sample prepa-ration techniques can be key to success of a proteomics experiment. Equally, lack of such knowledge can be the source of avoidable, failed experiments. Here, we will share our experience and discuss common contaminants such as buffers, solubilization reagents and those contaminants that are introduced through the use of specific biological protocols. The chapter will outline how best to deal with these common problems in order to maximize the returns from a proteomics experiment. We will also briefly discuss the importance of liquid chromatography and mass spectrometry optimization as well as bioinformatics tools to increase the amount of information that can be retrieved from such experiments.

Original languageEnglish
Title of host publicationBiomacromolecular Mass Spectrometry
Subtitle of host publicationTips from the Bench
PublisherNova Science Publishers, Inc
Pages19-34
Number of pages16
ISBN (Print)9781620819326
Publication statusPublished - 01 Nov 2012
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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