Azaspiracid: First evidence of protein binding in shellfish

K.J. Nzoughet; John Hamilton; Donna Floyd; Anne Douglas; John Nelson; Patricia Devine; Christopher Elliott

doi:10.1016/j.toxicon.2008.02.016

Azaspiracid: First evidence of protein binding in shellfish

K.J. Nzoughet, John Hamilton, Donna Floyd, Anne Douglas, John Nelson, Patricia Devine, Christopher Elliott

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

The occurrence of azaspiracid (AZA) toxins in contaminated shellfish has been the focus of much research. The present study investigated the binding properties of these toxins in mussels of the species Mytilus edulis. The work involved extraction of proteins and AZAs from contaminated mussel hepatopancreas and examination of the extracts by isoelectric focusing (IEF), size exclusion chromatography (SEC) and sodium docecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE). Liquid chromatography coupled with tandem mass spectrometry analysis (LC–MS/MS) was also performed in this study to identify AZAs. Blank mussels were subjected to the same purification and analytical procedures.

AZAs were found to be weakly bound to a protein with a molecular weight of 45 kDa, in samples of contaminated mussels. This protein, which was abundant in contaminated mussels, was also present in blank mussels, albeit at much lower concentrations. It was further noted that a 22 kDa protein was also present only in contaminated mussel samples.

Original language	English
Pages (from-to)	1255-1263
Number of pages	9
Journal	Toxicon
Volume	51
Issue number	7
DOIs	https://doi.org/10.1016/j.toxicon.2008.02.016
Publication status	Published - 01 Jun 2008

ASJC Scopus subject areas

Toxicology

Access to Document

10.1016/j.toxicon.2008.02.016

Cite this

@article{72eaf11241ce469cb99cbcb18350e6bf,

title = "Azaspiracid: First evidence of protein binding in shellfish",

abstract = "The occurrence of azaspiracid (AZA) toxins in contaminated shellfish has been the focus of much research. The present study investigated the binding properties of these toxins in mussels of the species Mytilus edulis. The work involved extraction of proteins and AZAs from contaminated mussel hepatopancreas and examination of the extracts by isoelectric focusing (IEF), size exclusion chromatography (SEC) and sodium docecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE). Liquid chromatography coupled with tandem mass spectrometry analysis (LC–MS/MS) was also performed in this study to identify AZAs. Blank mussels were subjected to the same purification and analytical procedures.AZAs were found to be weakly bound to a protein with a molecular weight of 45 kDa, in samples of contaminated mussels. This protein, which was abundant in contaminated mussels, was also present in blank mussels, albeit at much lower concentrations. It was further noted that a 22 kDa protein was also present only in contaminated mussel samples.",

author = "K.J. Nzoughet and John Hamilton and Donna Floyd and Anne Douglas and John Nelson and Patricia Devine and Christopher Elliott",

year = "2008",

month = jun,

day = "1",

doi = "10.1016/j.toxicon.2008.02.016",

language = "English",

volume = "51",

pages = "1255--1263",

journal = "Toxicon",

issn = "0041-0101",

publisher = "Elsevier Ltd",

number = "7",

}

TY - JOUR

T1 - Azaspiracid: First evidence of protein binding in shellfish

AU - Nzoughet, K.J.

AU - Hamilton, John

AU - Floyd, Donna

AU - Douglas, Anne

AU - Nelson, John

AU - Devine, Patricia

AU - Elliott, Christopher

PY - 2008/6/1

Y1 - 2008/6/1

N2 - The occurrence of azaspiracid (AZA) toxins in contaminated shellfish has been the focus of much research. The present study investigated the binding properties of these toxins in mussels of the species Mytilus edulis. The work involved extraction of proteins and AZAs from contaminated mussel hepatopancreas and examination of the extracts by isoelectric focusing (IEF), size exclusion chromatography (SEC) and sodium docecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE). Liquid chromatography coupled with tandem mass spectrometry analysis (LC–MS/MS) was also performed in this study to identify AZAs. Blank mussels were subjected to the same purification and analytical procedures.AZAs were found to be weakly bound to a protein with a molecular weight of 45 kDa, in samples of contaminated mussels. This protein, which was abundant in contaminated mussels, was also present in blank mussels, albeit at much lower concentrations. It was further noted that a 22 kDa protein was also present only in contaminated mussel samples.

AB - The occurrence of azaspiracid (AZA) toxins in contaminated shellfish has been the focus of much research. The present study investigated the binding properties of these toxins in mussels of the species Mytilus edulis. The work involved extraction of proteins and AZAs from contaminated mussel hepatopancreas and examination of the extracts by isoelectric focusing (IEF), size exclusion chromatography (SEC) and sodium docecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE). Liquid chromatography coupled with tandem mass spectrometry analysis (LC–MS/MS) was also performed in this study to identify AZAs. Blank mussels were subjected to the same purification and analytical procedures.AZAs were found to be weakly bound to a protein with a molecular weight of 45 kDa, in samples of contaminated mussels. This protein, which was abundant in contaminated mussels, was also present in blank mussels, albeit at much lower concentrations. It was further noted that a 22 kDa protein was also present only in contaminated mussel samples.

UR - http://www.scopus.com/inward/record.url?scp=43849103056&partnerID=8YFLogxK

U2 - 10.1016/j.toxicon.2008.02.016

DO - 10.1016/j.toxicon.2008.02.016

M3 - Article

C2 - 18417183

SN - 0041-0101

VL - 51

SP - 1255

EP - 1263

JO - Toxicon

JF - Toxicon

IS - 7

ER -

Azaspiracid: First evidence of protein binding in shellfish

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this