Bacteroidales recruit IL-6-producing intraepithelial lymphocytes in the colon to promote barrier integrity

KA Kuhn, HM Schulz, EH Regner, EL Severs, JD Hendrickson, G Mehta, AK Whitney, D Ir, N Ohri, CE Robertson, DN Frank, EL Campbell, SP Colgan

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31 Citations (Scopus)

Abstract

Interactions between the microbiota and distal gut are important for the maintenance of a healthy intestinal barrier; dysbiosis of intestinal microbial communities has emerged as a likely contributor to diseases that arise at the level of the mucosa. Intraepithelial lymphocytes (IELs) are positioned within the epithelial barrier, and in the small intestine they function to maintain epithelial homeostasis. We hypothesized that colon IELs promote epithelial barrier function through the expression of cytokines in response to interactions with commensal bacteria. Profiling of bacterial 16S ribosomal RNA revealed that candidate bacteria in the order Bacteroidales are sufficient to promote IEL presence in the colon that in turn produce interleukin-6 (IL-6) in a MyD88 (myeloid differentiation primary response 88)-dependent manner. IEL-derived IL-6 is functionally important in the maintenance of the epithelial barrier as IL-6−/− mice were noted to have increased paracellular permeability, decreased claudin-1 expression, and a thinner mucus gel layer, all of which were reversed by transfer of IL-6+/+ IELs, leading to protection of mice in response to Citrobacter rodentiuminfection. Therefore, we conclude that microbiota provide a homeostatic role for epithelial barrier function through regulation of IEL-derived IL-6.
Original languageEnglish
Pages (from-to)357-368
Number of pages12
JournalMucosal immunology
Volume11
Issue number2
Early online date16 Aug 2017
DOIs
Publication statusPublished - Mar 2018

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    Kuhn, KA., Schulz, HM., Regner, EH., Severs, EL., Hendrickson, JD., Mehta, G., Whitney, AK., Ir, D., Ohri, N., Robertson, CE., Frank, DN., Campbell, EL., & Colgan, SP. (2018). Bacteroidales recruit IL-6-producing intraepithelial lymphocytes in the colon to promote barrier integrity. Mucosal immunology, 11(2), 357-368. https://doi.org/10.1038/mi.2017.55