Biochemical and physiochemical characteristics of a pre-B cell stimulating factor produced by the plasmacytoma cell line LICR LON HMY2

A Trudgett, A C Fay, Jonathan Thompson, T A McNeill

Research output: Contribution to journalArticlepeer-review

Abstract

We have characterized the pre-B cell colony stimulating activity (pre-B cell CSA) from LICR LON HMY2 conditioned medium (CM) by a variety of biochemical techniques. Pre-B cell CSA was found to be associated with a heat stable glycoprotein which has an isoelectric point of 8.3 and a mol. wt, as determined by polyacrylamide gel electrophoresis, of 28-32 kD. The relationship of this activity to previously described factors acting on cells of the B cell lineage is discussed.
Original languageEnglish
Pages (from-to)637-43
Number of pages7
JournalClinical and experimental immunology
Volume59
Issue number3
Publication statusPublished - Mar 1985

Keywords

  • B-Lymphocytes
  • Cell Line
  • Cells, Cultured
  • Colony-Stimulating Factors
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Hydrolases
  • Isoelectric Point
  • Molecular Weight
  • Periodic Acid
  • Plasmacytoma
  • Potassium Compounds
  • Stem Cells

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