Biochemical and physiochemical characteristics of a pre-B cell stimulating factor produced by the plasmacytoma cell line LICR LON HMY2

A Trudgett; A C Fay; Jonathan Thompson; T A McNeill

Biochemical and physiochemical characteristics of a pre-B cell stimulating factor produced by the plasmacytoma cell line LICR LON HMY2

A Trudgett, A C Fay, Jonathan Thompson, T A McNeill

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

We have characterized the pre-B cell colony stimulating activity (pre-B cell CSA) from LICR LON HMY2 conditioned medium (CM) by a variety of biochemical techniques. Pre-B cell CSA was found to be associated with a heat stable glycoprotein which has an isoelectric point of 8.3 and a mol. wt, as determined by polyacrylamide gel electrophoresis, of 28-32 kD. The relationship of this activity to previously described factors acting on cells of the B cell lineage is discussed.

Original language	English
Pages (from-to)	637-43
Number of pages	7
Journal	Clinical and Experimental Immunology
Volume	59
Issue number	3
Publication status	Published - Mar 1985

Keywords

B-Lymphocytes
Cell Line
Cells, Cultured
Colony-Stimulating Factors
Electrophoresis, Polyacrylamide Gel
Humans
Hydrolases
Isoelectric Point
Molecular Weight
Periodic Acid
Plasmacytoma
Potassium Compounds
Stem Cells

Cite this

@article{914e7275bff0488e9e0a1f8cfc5abe4d,

title = "Biochemical and physiochemical characteristics of a pre-B cell stimulating factor produced by the plasmacytoma cell line LICR LON HMY2",

abstract = "We have characterized the pre-B cell colony stimulating activity (pre-B cell CSA) from LICR LON HMY2 conditioned medium (CM) by a variety of biochemical techniques. Pre-B cell CSA was found to be associated with a heat stable glycoprotein which has an isoelectric point of 8.3 and a mol. wt, as determined by polyacrylamide gel electrophoresis, of 28-32 kD. The relationship of this activity to previously described factors acting on cells of the B cell lineage is discussed.",

keywords = "B-Lymphocytes, Cell Line, Cells, Cultured, Colony-Stimulating Factors, Electrophoresis, Polyacrylamide Gel, Humans, Hydrolases, Isoelectric Point, Molecular Weight, Periodic Acid, Plasmacytoma, Potassium Compounds, Stem Cells",

author = "A Trudgett and Fay, {A C} and Jonathan Thompson and McNeill, {T A}",

year = "1985",

month = mar,

language = "English",

volume = "59",

pages = "637--43",

journal = "Clinical and Experimental Immunology",

issn = "0009-9104",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Biochemical and physiochemical characteristics of a pre-B cell stimulating factor produced by the plasmacytoma cell line LICR LON HMY2

AU - Trudgett, A

AU - Fay, A C

AU - Thompson, Jonathan

AU - McNeill, T A

PY - 1985/3

Y1 - 1985/3

N2 - We have characterized the pre-B cell colony stimulating activity (pre-B cell CSA) from LICR LON HMY2 conditioned medium (CM) by a variety of biochemical techniques. Pre-B cell CSA was found to be associated with a heat stable glycoprotein which has an isoelectric point of 8.3 and a mol. wt, as determined by polyacrylamide gel electrophoresis, of 28-32 kD. The relationship of this activity to previously described factors acting on cells of the B cell lineage is discussed.

AB - We have characterized the pre-B cell colony stimulating activity (pre-B cell CSA) from LICR LON HMY2 conditioned medium (CM) by a variety of biochemical techniques. Pre-B cell CSA was found to be associated with a heat stable glycoprotein which has an isoelectric point of 8.3 and a mol. wt, as determined by polyacrylamide gel electrophoresis, of 28-32 kD. The relationship of this activity to previously described factors acting on cells of the B cell lineage is discussed.

KW - B-Lymphocytes

KW - Cell Line

KW - Cells, Cultured

KW - Colony-Stimulating Factors

KW - Electrophoresis, Polyacrylamide Gel

KW - Humans

KW - Hydrolases

KW - Isoelectric Point

KW - Molecular Weight

KW - Periodic Acid

KW - Plasmacytoma

KW - Potassium Compounds

KW - Stem Cells

M3 - Article

C2 - 2985306

VL - 59

SP - 637

EP - 643

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 3

ER -

Biochemical and physiochemical characteristics of a pre-B cell stimulating factor produced by the plasmacytoma cell line LICR LON HMY2

Abstract

Keywords

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