Biochemical characterisation of a PL24 ulvan lyase from seaweed-associated Vibrio sp. FNV38

Valerie J. Rodrigues*, Diane Jouanneau, Narcis Fernandez-Fuentes, Lucy A. Onime, Sharon A. Huws, Annamma A. Odaneth, Jessica M. M. Adams

*Corresponding author for this work

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Abstract

Ulvan is a green macroalgal cell wall polysaccharide that has tremendous potential for valorisation due to its unique composition of sulphated rhamnose, glucuronic acid, iduronic acid and xylose. Several potential applications such as production of biofuels, bioplastics and other value-added products necessitate the breakdown of the polysaccharide to oligomers or monomers. Research on ulvan saccharifying enzymes has been continually increasing over the last decade, with the increasing focus on valorisation of seaweed biomass for a biobased economy. Lyases are the first of several enzymes that are involved in saccharifying the polysaccharide and several ulvan lyases have been structurally and biochemically characterised to enable their effective use in the valorisation processes. This study investigates the whole genome of Vibrio sp. FNV38, an ulvan metabolising organism and biochemical characteristics of a PL24 ulvan lyase that it possesses. The genome of Vibrio sp. FNV38 has a diverse CAZy profile with several genes involved in the metabolism of ulvan, cellulose, agar, and alginate. The enzyme exhibits optimal activity at pH 8.5 in 100 mM Tris–HCl buffer and 30 °C. However, its thermal stability is poor with significant loss of activity after 2 h of incubation at temperatures above 25 °C. Breakdown product analysis reveals that the enzyme depolymerised the polysaccharide predominantly to disaccharides and tetrasaccharides.
Original languageEnglish
Pages (from-to)697-711
Number of pages15
JournalJournal of Applied Phycology
Volume36
Issue number2
Early online date07 Dec 2023
DOIs
Publication statusPublished - 01 Apr 2024

Keywords

  • Polysaccharides
  • Vibrio
  • Macroalgae
  • Ulvan
  • Enzymes

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