Bioinspired nanozyme for portable immunoassay of allergenic proteins based on A smartphone

Xianlong Zhang, Di Wu, Yongning Wu, Guoliang Li*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)

Abstract

Inspired by the structure of the catalytically active center of natural laccase, a novel laccase mimics (named LM nanozymes) with a superior catalytic activity was successfully prepared by using glutathione (GSH) and copper (II) chloride as precursors via a facile hydrothermal method. The catalytically active center structure of LM nanozymes was revealed, which was constructed based on the numerous copper (Ⅰ) and copper (II) coordinating with thiol/amino group. The possible catalytic mechanism of LM nanozymes was also proposed. Similar to natural laccase, the prepared LM nanozymes can catalyze the oxidative coupling reaction between 2,4-dichlorophenol (2,4-DP) and 4-aminoantipyrine (4-AP) to produce an obvious red product. Compared with natural laccase, the LM nanozymes showed many outstanding advantages such as robust stability, lower cost, stronger catalytic activity and substrate affinity. Based on its excellent performances, LM nanozymes were employed as a powerful alternative to the natural enzyme in a traditional enzyme-linked immunosorbent assay (ELISA) to establish a nanozyme-based ELISA towards alpha-lactalbumin (allergenic protein). Impressively, a high-throughput and portable detection method was established by the integration of the nanozyme-based ELISA with a smartphone. The portable detection strategy achieved a limit of detection as low as 0.056 ng/mL with high specificity, and also showed excellent applicability in food sample analysis. This work not only enriches the diversities of nanozymes, but also broadens the promising applications of nanozymes in the biosensing area.

Original languageEnglish
Article number112776
JournalBiosensors and Bioelectronics
Volume172
Early online date04 Nov 2020
DOIs
Publication statusPublished - 15 Jan 2021

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (32022069 and 31801454), the Science and Technology Nova Plan of Shaanxi Province (2019KJXX-010), Newton International Fellowships programme of RoyalSociety NIF\R1\192293, the Project funded by China Postdoctoral Science Foundation (2018 M640574), and the Youth Innovation Team of Shaanxi Universities (Food Quality and Safety).

Funding Information:
This work was supported by the National Natural Science Foundation of China ( 32022069 and 31801454 ), the Science and Technology Nova Plan of Shaanxi Province ( 2019KJXX-010 ), Newton International Fellowships programme of RoyalSociety NIF\R1\192293, the Project funded by China Postdoctoral Science Foundation ( 2018 M640574 ), and the Youth Innovation Team of Shaanxi Universities (Food Quality and Safety).

Publisher Copyright:
© 2020

Keywords

  • Allergenic protein assay
  • Biosensing
  • Enzyme-linked immunosorbent assay
  • Nanozymes
  • Smartphone

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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