Bisphosphonate ligand mediated ultrasensitive capacitive protein sensor: complementary match of supramolecular and dynamic chemistry

Gizem Ertürk, Maedeh Akhoundian, Kyra Lueg-Althoff, Sudhirkumar Shinde, Sing Yee Yeung, Martin Hedström, Thomas Schrader, Bo Mattiasson, Börje Sellergren

Research output: Contribution to journalArticle

Abstract

A powerful polymeric protein sensor was constructed by microcontact imprinting taking advantage of the specific interaction between a bisphosphonate binding monomer and lysine/arginine residues on the surface of trypsin.Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the specific interaction between bisphosphonate binding monomers and lysine/arginine residues on the surface of trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We believe that the combination of supramolecular chemistry, molecular imprinting and advanced instrumentation has a potential for future drug development and diagnostics that extends beyond biomolecular recognition.
Original languageEnglish
Pages (from-to)847-852
Number of pages6
JournalNew journal of chemistry
Volume43
Issue number2
Early online date04 Dec 2018
DOIs
Publication statusPublished - 14 Jan 2019

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Arginine
Diphosphonates
Trypsin
Monomers
Ligands
Proteins
Sensors
Supramolecular chemistry
Lysine
Capacitive sensors
Ionic strength
Enzymes
Doping (additives)
Buffers
Pharmaceutical Preparations

Cite this

Ertürk, Gizem ; Akhoundian, Maedeh ; Lueg-Althoff, Kyra ; Shinde, Sudhirkumar ; Yeung, Sing Yee ; Hedström, Martin ; Schrader, Thomas ; Mattiasson, Bo ; Sellergren, Börje. / Bisphosphonate ligand mediated ultrasensitive capacitive protein sensor: complementary match of supramolecular and dynamic chemistry. In: New journal of chemistry. 2019 ; Vol. 43, No. 2. pp. 847-852.
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Ertürk, G, Akhoundian, M, Lueg-Althoff, K, Shinde, S, Yeung, SY, Hedström, M, Schrader, T, Mattiasson, B & Sellergren, B 2019, 'Bisphosphonate ligand mediated ultrasensitive capacitive protein sensor: complementary match of supramolecular and dynamic chemistry', New journal of chemistry, vol. 43, no. 2, pp. 847-852. https://doi.org/10.1039/c8nj05238g

Bisphosphonate ligand mediated ultrasensitive capacitive protein sensor: complementary match of supramolecular and dynamic chemistry. / Ertürk, Gizem; Akhoundian, Maedeh; Lueg-Althoff, Kyra; Shinde, Sudhirkumar; Yeung, Sing Yee; Hedström, Martin; Schrader, Thomas; Mattiasson, Bo; Sellergren, Börje.

In: New journal of chemistry, Vol. 43, No. 2, 14.01.2019, p. 847-852.

Research output: Contribution to journalArticle

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AU - Akhoundian, Maedeh

AU - Lueg-Althoff, Kyra

AU - Shinde, Sudhirkumar

AU - Yeung, Sing Yee

AU - Hedström, Martin

AU - Schrader, Thomas

AU - Mattiasson, Bo

AU - Sellergren, Börje

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