BopC is a type III secreted effector protein of Burkholderia pseudomallei

Sunsiree Muangman, Sunee Korbsrisate, Veerachat Muangsombut, Varintip Srinon, Natalie Lazar Adler, Gunnar N. Schroeder, Gad Frankel, Edouard E. Galyov*

*Corresponding author for this work

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Burkholderia pseudomallei, the causative agent of melioidosis, exploits the Bsa type III secretion system (T3SS) to deliver effector proteins into host cells. These effectors manipulate host cell functions; thus, contributing to the ability of the bacteria to evade the immune response and cause disease. Only two Bsa-secreted effectors have been conclusively identified to date. Here, we report the identification of the third B. pseudomallei type III secreted effector protein, designated BopC. BopC is encoded by the bpss1516 gene abutting bpss1517, which encodes its putative chaperone. The genes are located in the close proximity to the bsa T3SS gene cluster of B. pseudomallei K96243 (Fig. 1). BopC was secreted into culture supernatant by the wild-type B. pseudomallei strain, but its secretion was abolished in the bsaZ T3SS mutant. Using pull down and co-purification assays, we confirmed that BopC interacts with its putative chaperone, BPSS1517, in vitro. Furthermore, the first 20 N-terminal amino acids of BopC were found to be sufficient to mediate the T3SS-dependent translocation of a reporter protein from a heterologous enteropathogenic Escherichia coli host into mammalian cells. Finally, bopC mutant was found to be less invasive than the wild-type strain in the epithelial cells.

Original languageEnglish
Pages (from-to)75-82
Number of pages8
JournalFEMS Microbiology Letters
Volume323
Issue number1
DOIs
Publication statusPublished - Oct 2011
Externally publishedYes

Keywords

  • BopC
  • Bsa T3SS
  • Burkholderia pseudomallei
  • Effector protein

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

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  • Cite this

    Muangman, S., Korbsrisate, S., Muangsombut, V., Srinon, V., Adler, N. L., Schroeder, G. N., Frankel, G., & Galyov, E. E. (2011). BopC is a type III secreted effector protein of Burkholderia pseudomallei. FEMS Microbiology Letters, 323(1), 75-82. https://doi.org/10.1111/j.1574-6968.2011.02359.x