Abstract
A novel, competitive immunoassay based on time-resolved fluorimetry was developed, and used to measure the serum concentration of bovine transferrin during acute Haemophilus somnus pneumonia. Upper and lower limits of normality were established using serum from healthy cattle (3.72-1.37mg mL-1). Following experimental infection with Haemophilus somnus, transferrin concentration was depressed in all calves but recovered to pre-infection levels in groups of calves which had either no lesions, or mild lesions at necropsy between 5 and 6 days after infection. In a third group, which developed extensive lesions, the transferrin concentration remained depressed. Transferrin levels remained within the normal range for all calves during the experimental period. Those calves which had low transferrin concentrations pre-infection, developed extensive lung lesions following experimental infection with Haemophilus somnus.
| Original language | English |
|---|---|
| Pages (from-to) | 251-255 |
| Number of pages | 5 |
| Journal | Veterinary Journal |
| Volume | 155 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 01 Jan 1998 |
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Keywords
- Bovine transferrin
- Competitive immunoassay
- Haemophilus somnus
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Bovine serum transferrin concentration during acute infection with Haemophilus somnus. / McNair, J.; Elliott, C.; Bryson, D. G.; Mackie, D. P.
In: Veterinary Journal, Vol. 155, No. 3, 01.01.1998, p. 251-255.Research output: Contribution to journal › Article
TY - JOUR
T1 - Bovine serum transferrin concentration during acute infection with Haemophilus somnus
AU - McNair, J.
AU - Elliott, C.
AU - Bryson, D. G.
AU - Mackie, D. P.
PY - 1998/1/1
Y1 - 1998/1/1
N2 - A novel, competitive immunoassay based on time-resolved fluorimetry was developed, and used to measure the serum concentration of bovine transferrin during acute Haemophilus somnus pneumonia. Upper and lower limits of normality were established using serum from healthy cattle (3.72-1.37mg mL-1). Following experimental infection with Haemophilus somnus, transferrin concentration was depressed in all calves but recovered to pre-infection levels in groups of calves which had either no lesions, or mild lesions at necropsy between 5 and 6 days after infection. In a third group, which developed extensive lesions, the transferrin concentration remained depressed. Transferrin levels remained within the normal range for all calves during the experimental period. Those calves which had low transferrin concentrations pre-infection, developed extensive lung lesions following experimental infection with Haemophilus somnus.
AB - A novel, competitive immunoassay based on time-resolved fluorimetry was developed, and used to measure the serum concentration of bovine transferrin during acute Haemophilus somnus pneumonia. Upper and lower limits of normality were established using serum from healthy cattle (3.72-1.37mg mL-1). Following experimental infection with Haemophilus somnus, transferrin concentration was depressed in all calves but recovered to pre-infection levels in groups of calves which had either no lesions, or mild lesions at necropsy between 5 and 6 days after infection. In a third group, which developed extensive lesions, the transferrin concentration remained depressed. Transferrin levels remained within the normal range for all calves during the experimental period. Those calves which had low transferrin concentrations pre-infection, developed extensive lung lesions following experimental infection with Haemophilus somnus.
KW - Bovine transferrin
KW - Competitive immunoassay
KW - Haemophilus somnus
UR - http://www.scopus.com/inward/record.url?scp=0032060093&partnerID=8YFLogxK
U2 - 10.1016/S1090-0233(05)80020-6
DO - 10.1016/S1090-0233(05)80020-6
M3 - Article
C2 - 9638071
AN - SCOPUS:0032060093
VL - 155
SP - 251
EP - 255
JO - The Veterinary Journal
JF - The Veterinary Journal
SN - 1090-0233
IS - 3
ER -