TY - JOUR
T1 - Bradykinin-related peptides, including a novel structural variant, (Val1)-bradykinin, from the skin secretion of Guenther's frog, Hylarana guentheri and their molecular precursors.
AU - Zhou, J.
AU - Bjourson, A.J.
AU - Coulter, D.J.M.
AU - Chen, Tianbao
AU - Shaw, Christopher
AU - O’Rourke, M.
AU - Hirst, David
AU - Zhang, Y.
AU - Rao, P.F.
AU - McClean, S.
PY - 2007/4
Y1 - 2007/4
N2 - Multiple bradykinin-related peptides including a novel bradykinin structural variant, (Val1)-bradykinin, have been identified from the defensive skin secretion of Guenther's frog, Hylarana guentheri by a tandem mass spectrometry method. Subsequently, four different preprobradykinin cDNAs, which encoded multiple bradykinin copies and its structural variants, were consistently cloned from a skin derived cDNA library. These preprobradykinin cDNAs showed little structural similarity with mammalian kininogens and the kininogens from the skin of toads, but have regions that are highly conserved in the kininogens from another ranid frog, Odorrana schmackeri. Alignment of these preprobradykinins revealed that preprobradykinin 1, 2 and 3 may derive from a single gene by alternative exon splicing.
AB - Multiple bradykinin-related peptides including a novel bradykinin structural variant, (Val1)-bradykinin, have been identified from the defensive skin secretion of Guenther's frog, Hylarana guentheri by a tandem mass spectrometry method. Subsequently, four different preprobradykinin cDNAs, which encoded multiple bradykinin copies and its structural variants, were consistently cloned from a skin derived cDNA library. These preprobradykinin cDNAs showed little structural similarity with mammalian kininogens and the kininogens from the skin of toads, but have regions that are highly conserved in the kininogens from another ranid frog, Odorrana schmackeri. Alignment of these preprobradykinins revealed that preprobradykinin 1, 2 and 3 may derive from a single gene by alternative exon splicing.
UR - http://www.scopus.com/inward/record.url?scp=33847372913&partnerID=8YFLogxK
U2 - 10.1016/j.peptides.2007.01.014
DO - 10.1016/j.peptides.2007.01.014
M3 - Article
SN - 0196-9781
VL - 28 (4)
SP - 781
EP - 789
JO - Peptides
JF - Peptides
IS - 4
ER -