Bradykinin and (Thr6)-bradykinin have been identified in the defensive skin secretion of the fire-bellied toad, Bombina orientalis. The homologous cDNAs for both peptides were cloned from a skin library using a 3'- and 5'-RACE strategy. Kininogen-1 (BOK-1) contained an open-reading frame of 167 amino acid residues encoding four repeats of bradykinin, and kininogen-2 (BOK-2) contained an open-reading frame of 161 amino acid residues encoding two repeats of (Thr6)-bradykinin. Alignment of both precursor nucleotide and amino acid sequences revealed a high degree of structural similarity. These amphibian skin kininogens/preprobradykinins are not biologically analogous to mammalian kininogens.
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
Chen, T., Orr, D. F., Bjourson, A. J., McClean, S., O'Rourke, M., Hirst, D., Rao, P. F., & Shaw, C. (2002). Bradykinins and their precursor Cdnas from the skin of the Fire-Bellied Toad (Bombina orientalis). Peptides, 23(9), 1547-1555. https://doi.org/10.1016/S0196-9781(02)00095-5