Abstract
Host-defence antimicrobial peptides (AMPs) from amphibians are usually considered as one of the most promising next-generation antibiotics because of their excellent antimicrobial properties and low cytotoxicity. In this study, one novel Brevinin-1 type peptide, Brevinin-1GHd, was isolated and characterized from the skin secretion of the frog, Hylarana guentheri. Brevinin-1GHd was found to possess a wide range of antimicrobial activity through penetrating the bacterial membrane within a short time while showing low hemolysis at bactericidal concentrations, even against the resistant strains. It also inhibited and eradicated biofilms which are thought to be closely related to the rise in resistance. Meanwhile, Brevinin-1GHd exhibited wide-spectrum anti-proliferation activity toward human cancer lines. Taken together, these results indicate that Brevinin-1GHd with its excellent antimicrobial and anticancer activities, is a promising candidate for a novel antibiotic agent, and study of its structure-activity relationships also provided a rational template for further research and peptide analog design.
Original language | English |
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Article number | BSR20200019 |
Number of pages | 15 |
Journal | Bioscience Reports |
Volume | 40 |
Issue number | 5 |
Early online date | 29 Apr 2020 |
DOIs | |
Publication status | Published - 14 May 2020 |
Keywords
- Brevinin-1GHd; anti-proliferation; antimicrobial peptides (AMPs); biofilms; resistant strains
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Dive into the research topics of 'Brevinin-1GHd, A Novel Hylarana guentheri Skin Secretion-Derived Brevinin-1 Type Peptide with Antimicrobial and Anticancer Therapeutic Potential'. Together they form a unique fingerprint.Student theses
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Characterisation, bioactivity evaluation and rational modification of bioactive peptides from frog skin secretion and scorpion venom
Author: Wang, T., Jul 2021Supervisor: Zhou, M. (Supervisor), Chen, X. (Supervisor), Chen, T. (Supervisor) & Wang, L. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy
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The membrane-active antimicrobial peptide from frog skin and scorpion venom: From mechanistic insight to design
Author: Jiang, Y., Jul 2021Supervisor: Wang, L. (Supervisor), Chen, X. (Supervisor), Chen, T. (Supervisor) & Zhou, M. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy