Burkholderia cenocepacia O polysaccharide chain contributes to caspase-1-dependent IL-1 beta production in macrophages

S. Kotrange, B. Kopp, A. Akhter, D. Abdelaziz, A. Abu Khweek, K. Caution, B. Abdulrahman, M.D. Wewers, K. Mccoy, C. Marsh, S.A. Loutet, X. Ortega, Miguel Valvano, A.O. Amer

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Burkholderia cenocepacia infections in CF patients involve heightened inflammation, fatal sepsis, and high antibiotic resistance. Proinflammatory IL-1 beta secretion is important in airway inflammation and tissue damage. However, little is known about this pathway in macrophages upon B. cenocepacia infection. We report here that murine macrophages infected with B. cenocepacia K56-2 produce proinflammatory cytokine IL-1 beta in a TLR4 and caspase-1-mediated manner. We also determined that the OPS (O antigen) of B. cenocepacia LPS contributes to IL-1 beta production and pyroptotic cell death. Furthermore, we showed that the malfunction of the CFTR channel augmented IL-1 beta production upon B. cenocepacia infection of murine macrophages. Taken together, we identified eukaryotic and bacterial factors that contribute to inflammation during B. cenocepacia infection, which may aid in the design of novel approaches to control pulmonary inflammation. J. Leukoc. Biol. 89: 481-488; 2011.
Original languageEnglish
Pages (from-to)481-488
Number of pages8
JournalJournal of Leukocyte Biology
Volume89
Issue number3
DOIs
Publication statusPublished - Mar 2011

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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