Capillary Electrophoresis of Erythrocytes.

W.H. Lu, W.H. Deng, S.T. Liu, Tianbao Chen, P.F. Rao

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Capillary electrophoresis (CE) of erythrocytes from different sources under various conditions is reported in this paper. It was found that erythrocyte samples from sheep, duck, and human showed characteristic and reproducible elution peaks, and that the retention times of A-, B-, AB-, and O-type erythrocytes from human blood were distinctively different; even subtle differences, among individuals with the same blood type could be detected by CE. A strictly linear correlation was obtained between the peak area and the amount of human erythrocyte over a range of 4.8×102–1.9×104 cells (r=0.999), indicating that CE could be used for rapid and accurate quantification of erythrocytes. Using this CE protocol, the decrease of the surface electrical charge of erythrocyte during storage was confirmed. Therefore, this work demonstrated that CE could be a useful alternative for characterizing and quantifying erythrocytes or other cells.
Original languageEnglish
Pages (from-to)194-198
Number of pages5
JournalAnalytical Biochemistry
Volume314 (2)
Issue number2
DOIs
Publication statusPublished - 15 Mar 2003

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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