Carbon-phosphorus bond cleavage activity in cell-free extracts of enterobacter aerogenes ATCC 15038 and pseudomonas sp. 4ASW

G. McMullan; R. Watkins; D. B. Harper; J. P. Quinn

Carbon-phosphorus bond cleavage activity in cell-free extracts of enterobacter aerogenes ATCC 15038 and pseudomonas sp. 4ASW

G. McMullan^*, R. Watkins, D. B. Harper, J. P. Quinn

^*Corresponding author for this work

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19 Citations (Scopus)

Abstract

Carbon-phosphorus bond cleavage activity was investigated in cell-free extracts of Enterobacter aerogenes ATCC 15038 (IFO 12010) and Pseudomonas sp. 4ASW, strains known to utilize a range of phosphonates as sole phosphorus source. In vitro phosphonatase activity was detected in extracts of both organisms, however extensive analysis failed to detect any organic product from phosphonates other than phosphonoacetaldehyde. Non-specific liberation of phosphate was observed in Pseudomonas sp. 4ASW, associated with a single fraction of FPLC-purified extract, and is believed to result from the activity of cellular phosphatases.

Original language	English
Pages (from-to)	271-279
Number of pages	9
Journal	Biochemistry International
Volume	25
Issue number	2
Publication status	Published - 1991

ASJC Scopus subject areas

Biochemistry

Cite this

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title = "Carbon-phosphorus bond cleavage activity in cell-free extracts of enterobacter aerogenes ATCC 15038 and pseudomonas sp. 4ASW",

abstract = "Carbon-phosphorus bond cleavage activity was investigated in cell-free extracts of Enterobacter aerogenes ATCC 15038 (IFO 12010) and Pseudomonas sp. 4ASW, strains known to utilize a range of phosphonates as sole phosphorus source. In vitro phosphonatase activity was detected in extracts of both organisms, however extensive analysis failed to detect any organic product from phosphonates other than phosphonoacetaldehyde. Non-specific liberation of phosphate was observed in Pseudomonas sp. 4ASW, associated with a single fraction of FPLC-purified extract, and is believed to result from the activity of cellular phosphatases.",

author = "G. McMullan and R. Watkins and Harper, {D. B.} and Quinn, {J. P.}",

year = "1991",

language = "English",

volume = "25",

pages = "271--279",

journal = "Biochemistry International",

issn = "0158-5231",

publisher = "Academic Press Inc.",

number = "2",

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T1 - Carbon-phosphorus bond cleavage activity in cell-free extracts of enterobacter aerogenes ATCC 15038 and pseudomonas sp. 4ASW

AU - McMullan, G.

AU - Watkins, R.

AU - Harper, D. B.

AU - Quinn, J. P.

PY - 1991

Y1 - 1991

N2 - Carbon-phosphorus bond cleavage activity was investigated in cell-free extracts of Enterobacter aerogenes ATCC 15038 (IFO 12010) and Pseudomonas sp. 4ASW, strains known to utilize a range of phosphonates as sole phosphorus source. In vitro phosphonatase activity was detected in extracts of both organisms, however extensive analysis failed to detect any organic product from phosphonates other than phosphonoacetaldehyde. Non-specific liberation of phosphate was observed in Pseudomonas sp. 4ASW, associated with a single fraction of FPLC-purified extract, and is believed to result from the activity of cellular phosphatases.

AB - Carbon-phosphorus bond cleavage activity was investigated in cell-free extracts of Enterobacter aerogenes ATCC 15038 (IFO 12010) and Pseudomonas sp. 4ASW, strains known to utilize a range of phosphonates as sole phosphorus source. In vitro phosphonatase activity was detected in extracts of both organisms, however extensive analysis failed to detect any organic product from phosphonates other than phosphonoacetaldehyde. Non-specific liberation of phosphate was observed in Pseudomonas sp. 4ASW, associated with a single fraction of FPLC-purified extract, and is believed to result from the activity of cellular phosphatases.

UR - http://www.scopus.com/inward/record.url?scp=0026339789&partnerID=8YFLogxK

M3 - Article

C2 - 1789794

AN - SCOPUS:0026339789

SN - 0158-5231

VL - 25

SP - 271

EP - 279

JO - Biochemistry International

JF - Biochemistry International

IS - 2

ER -

Carbon-phosphorus bond cleavage activity in cell-free extracts of enterobacter aerogenes ATCC 15038 and pseudomonas sp. 4ASW

Abstract

ASJC Scopus subject areas

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