Abstract
Effector caspases-3, -6 and -7 are responsible for producing the morphological features associated with apoptosis, such as DNA fragmentation. The present study demonstrates that a member of a novel series of pyrrolo-1,5-benzoxazepines, PBOX-6, induces apoptosis in MCF-7 cells, which lack caspase-3. Apoptosis was accompanied by DNA fragmentation and the activation of caspase-7, but not caspases-3 and -6. Inhibition of caspase-7 activity reduced the extent of apoptosis induced, indicating that activation of caspase-7 is involved in the mechanism by which PBOX-6 induces apoptosis in MCF-7 cells. This study suggests that caspase-3 is not necessarily essential for DNA fragmentation and the morphological changes associated with apoptosis.
Original language | English |
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Pages (from-to) | 66-70 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 515 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - 27 Mar 2002 |
Externally published | Yes |
Keywords
- Apoptosis
- Caspase
- DNA fragmentation
- MCF-7
- Pyrrolo-1,5-benzoxazepine
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology