Catalytic and biophysical investigation of rhodium hydroformylase

Hasan Tanvir Imam, Amanda Jarvis, Veronica Celorrio, Irshad Baig, Christopher Allen, Andrew Marr, Paul Kamer

Research output: Contribution to journalArticle

Abstract

Rh-containing artificial metalloenzymes based on two mutants of Sterol Carrier Protein_2L (SCP_2L) have been shown to act as hydroformylases, exhibiting significant activity and unexpectedly high selectivity in the hydroformylation of a range of alkenes. Here we report modifications of the catalyst performance by site directed mutagenesis, and studies of the biophysical properties of these catalysts. Catalysts were prepared based on single methionine mutants of SCP_2L for hydroformylation studies. Multinuclear (1H, & 13C), multi-dimensional (2D) solution NMR spectroscopy, EXAFS and XANES were employed to probe the structure. Biophysical studies using 2D [1H 13C] HSQC NMR spectroscopy of 13C-methyl methionine labeled catalysts were used to investigate changes in protein conformation. Hydroformylation studies employing the methionine mutants as catalysts revealed the significant effects of mutation on hydroformylation activity. Among the methionine mutant catalysts M1A of V83C and A100C, and M112A of V83C exhibited significantly higher activity than their parent proteins with improved selectivity. A metal binding role for methionine was suggested by EXAFS and XANES data on selenomethionine variants.
Original languageEnglish
JournalCATALYSIS SCIENCE & TECHNOLOGY
Early online date16 Oct 2019
DOIs
Publication statusEarly online date - 16 Oct 2019

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Rhodium
Hydroformylation
Methionine
Catalysts
Nuclear magnetic resonance spectroscopy
Selenomethionine
Proteins
Mutagenesis
Alkenes
Olefins
Conformations
Metals

Cite this

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title = "Catalytic and biophysical investigation of rhodium hydroformylase",
abstract = "Rh-containing artificial metalloenzymes based on two mutants of Sterol Carrier Protein_2L (SCP_2L) have been shown to act as hydroformylases, exhibiting significant activity and unexpectedly high selectivity in the hydroformylation of a range of alkenes. Here we report modifications of the catalyst performance by site directed mutagenesis, and studies of the biophysical properties of these catalysts. Catalysts were prepared based on single methionine mutants of SCP_2L for hydroformylation studies. Multinuclear (1H, & 13C), multi-dimensional (2D) solution NMR spectroscopy, EXAFS and XANES were employed to probe the structure. Biophysical studies using 2D [1H 13C] HSQC NMR spectroscopy of 13C-methyl methionine labeled catalysts were used to investigate changes in protein conformation. Hydroformylation studies employing the methionine mutants as catalysts revealed the significant effects of mutation on hydroformylation activity. Among the methionine mutant catalysts M1A of V83C and A100C, and M112A of V83C exhibited significantly higher activity than their parent proteins with improved selectivity. A metal binding role for methionine was suggested by EXAFS and XANES data on selenomethionine variants.",
author = "Imam, {Hasan Tanvir} and Amanda Jarvis and Veronica Celorrio and Irshad Baig and Christopher Allen and Andrew Marr and Paul Kamer",
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Catalytic and biophysical investigation of rhodium hydroformylase. / Imam, Hasan Tanvir; Jarvis, Amanda; Celorrio, Veronica; Baig, Irshad; Allen, Christopher; Marr, Andrew; Kamer, Paul.

In: CATALYSIS SCIENCE & TECHNOLOGY, 16.10.2019.

Research output: Contribution to journalArticle

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T1 - Catalytic and biophysical investigation of rhodium hydroformylase

AU - Imam, Hasan Tanvir

AU - Jarvis, Amanda

AU - Celorrio, Veronica

AU - Baig, Irshad

AU - Allen, Christopher

AU - Marr, Andrew

AU - Kamer, Paul

PY - 2019/10/16

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N2 - Rh-containing artificial metalloenzymes based on two mutants of Sterol Carrier Protein_2L (SCP_2L) have been shown to act as hydroformylases, exhibiting significant activity and unexpectedly high selectivity in the hydroformylation of a range of alkenes. Here we report modifications of the catalyst performance by site directed mutagenesis, and studies of the biophysical properties of these catalysts. Catalysts were prepared based on single methionine mutants of SCP_2L for hydroformylation studies. Multinuclear (1H, & 13C), multi-dimensional (2D) solution NMR spectroscopy, EXAFS and XANES were employed to probe the structure. Biophysical studies using 2D [1H 13C] HSQC NMR spectroscopy of 13C-methyl methionine labeled catalysts were used to investigate changes in protein conformation. Hydroformylation studies employing the methionine mutants as catalysts revealed the significant effects of mutation on hydroformylation activity. Among the methionine mutant catalysts M1A of V83C and A100C, and M112A of V83C exhibited significantly higher activity than their parent proteins with improved selectivity. A metal binding role for methionine was suggested by EXAFS and XANES data on selenomethionine variants.

AB - Rh-containing artificial metalloenzymes based on two mutants of Sterol Carrier Protein_2L (SCP_2L) have been shown to act as hydroformylases, exhibiting significant activity and unexpectedly high selectivity in the hydroformylation of a range of alkenes. Here we report modifications of the catalyst performance by site directed mutagenesis, and studies of the biophysical properties of these catalysts. Catalysts were prepared based on single methionine mutants of SCP_2L for hydroformylation studies. Multinuclear (1H, & 13C), multi-dimensional (2D) solution NMR spectroscopy, EXAFS and XANES were employed to probe the structure. Biophysical studies using 2D [1H 13C] HSQC NMR spectroscopy of 13C-methyl methionine labeled catalysts were used to investigate changes in protein conformation. Hydroformylation studies employing the methionine mutants as catalysts revealed the significant effects of mutation on hydroformylation activity. Among the methionine mutant catalysts M1A of V83C and A100C, and M112A of V83C exhibited significantly higher activity than their parent proteins with improved selectivity. A metal binding role for methionine was suggested by EXAFS and XANES data on selenomethionine variants.

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