Cataract surgery induces retinal pro-inflammatory gene expression and protein secretion

Heping Xu, Mei Chen, J.V. Forrester, N. Loin

Research output: Contribution to journalArticlepeer-review

63 Citations (Scopus)

Abstract

To investigate the effect(s) of cataract surgery on the expression of pro-inflammatory genes and proteins in the retina using an experimental rodent model. An extracapsular lens extraction was performed in one eye of C57BL/6 mice (n=24); the contralateral unoperated eyes (n =24) as well as eyes from unoperated animals (n = 9) served as controls. The neurosensory retina and retinal pigment epithelium (RPE)/choroid were collected postoperatively. Expression of genes involved in the acute inflammatory/ injury response, including IL-1ß, fibroblast growth factor, transforming growth factor ß, chemokine CCL2, SDF-1, and complements C3, C4, and factor B (CFB), were examined by real-time PCR and, selectively, by immunohistochemistry. The expression of IL-1 ß and CCL2 genes was markedly upregulated (>0-fold, P >0.01) in the neurosensory retina 30 minutes postoperatively and maintained for the 2-week postoperative period of observation; IL-1 ß expression was also upregulated in RPE/choroid. The expression of complement C3 (>-fold) and CFB (>0-fold) genes in the neurosensory retina was also significantly upregulated (P
Original languageEnglish
Pages (from-to)249-255
Number of pages7
JournalInvestigative Opthalmology and Visual Science
Volume52
Issue number1
DOIs
Publication statusPublished - Jan 2011

ASJC Scopus subject areas

  • Medicine(all)
  • Ophthalmology
  • Cellular and Molecular Neuroscience
  • Sensory Systems

Fingerprint Dive into the research topics of 'Cataract surgery induces retinal pro-inflammatory gene expression and protein secretion'. Together they form a unique fingerprint.

Cite this