Cationic Liposome- Multi-Walled Carbon Nanotubes Hybrids for Dual siPLK1 and Doxorubicin Delivery In Vitro

Sara Pereira; Jin Lee; Noelia Rubio; Hatem A F M Hassan; Izzat Bin Mohamed Suffian; Julie T-W Wang; Rebecca Klippstein; Belén Ballesteros; Wafa' T Al-Jamal; Khuloud T Al-Jamal

doi:10.1007/s11095-015-1707-1

Cationic Liposome- Multi-Walled Carbon Nanotubes Hybrids for Dual siPLK1 and Doxorubicin Delivery In Vitro

Sara Pereira, Jin Lee, Noelia Rubio, Hatem A F M Hassan, Izzat Bin Mohamed Suffian, Julie T-W Wang, Rebecca Klippstein, Belén Ballesteros, Wafa' T Al-Jamal, Khuloud T Al-Jamal

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

PURPOSE

To formulate f-MWNTs-cationic liposome hybrids for the simultaneous delivery of siPLK1 and doxorubicin to cancer cells.

METHOD

f-MWNTs-cationic liposome hybrids were prepared by the thin film hydration method where the lipid film was hydrated with 100 μg/ml or 1 mg/ml of ox-MWNTs-NH3 (+) or MWNTs-NH3 (+) in 5% dextrose. siRNA complexation and protection ability was determined by agarose gel electrophoresis. f-MWNTs and liposome interaction was evaluated using Nile Red (NR) fluorescence spectroscopy. Cellular uptake in A549 cells was assessed by flow cytometry. Silencing of target proteins was determined by Luciferase and MTT assays. Sub-G1 analysis was performed to evaluate apoptosis following co-delivery of siPLK1 and Doxorubicin (Dox).

RESULTS

Zeta potential and siRNA complexation profile obtained for all hybrids were comparable to those achieved with cationic liposomes. ox-MWNTs-NH3 (+) showed greater extent of interaction with cationic liposomes compared to MWNTs-NH3 (+). ox-MWNTs-NH3 (+) was able to protect siRNA from nuclease-mediated degradation. Enhanced cellular uptake of both the carrier and loaded siRNA in A549 cell, were observed for this hybrid compared to the liposomal carrier. A synergistic pro-apoptotic effect was obtained when siPLK1 silencing was combined with doxorubicin treatment for the hybrid:siRNA complexes compared to the lipoplexes, in A549 cells in vitro.

CONCLUSIONS

f-MWNTs-cationic liposome hybrid designed in this study can serve as a potential vehicle for the co-delivery of siRNA and cytotoxic drugs to cancer cells in vitro.

Original language	English
Pages (from-to)	3293-308
Journal	Pharmaceutical Research
Volume	32
Issue number	10
Early online date	18 Jun 2015
DOIs	https://doi.org/10.1007/s11095-015-1707-1
Publication status	Published - Oct 2015
Externally published	Yes

Keywords

Ammonium Compounds
Antineoplastic Agents
Apoptosis
Cations
Cell Line, Tumor
Chemistry, Pharmaceutical
Doxorubicin
Drug Delivery Systems
Humans
Liposomes
Nanotubes, Carbon
RNA, Small Interfering
Journal Article
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{43493810763e44e8942347992287df86,

title = "Cationic Liposome- Multi-Walled Carbon Nanotubes Hybrids for Dual siPLK1 and Doxorubicin Delivery In Vitro",

abstract = "PURPOSETo formulate f-MWNTs-cationic liposome hybrids for the simultaneous delivery of siPLK1 and doxorubicin to cancer cells.METHOD f-MWNTs-cationic liposome hybrids were prepared by the thin film hydration method where the lipid film was hydrated with 100 μg/ml or 1 mg/ml of ox-MWNTs-NH3 (+) or MWNTs-NH3 (+) in 5% dextrose. siRNA complexation and protection ability was determined by agarose gel electrophoresis. f-MWNTs and liposome interaction was evaluated using Nile Red (NR) fluorescence spectroscopy. Cellular uptake in A549 cells was assessed by flow cytometry. Silencing of target proteins was determined by Luciferase and MTT assays. Sub-G1 analysis was performed to evaluate apoptosis following co-delivery of siPLK1 and Doxorubicin (Dox).RESULTS Zeta potential and siRNA complexation profile obtained for all hybrids were comparable to those achieved with cationic liposomes. ox-MWNTs-NH3 (+) showed greater extent of interaction with cationic liposomes compared to MWNTs-NH3 (+). ox-MWNTs-NH3 (+) was able to protect siRNA from nuclease-mediated degradation. Enhanced cellular uptake of both the carrier and loaded siRNA in A549 cell, were observed for this hybrid compared to the liposomal carrier. A synergistic pro-apoptotic effect was obtained when siPLK1 silencing was combined with doxorubicin treatment for the hybrid:siRNA complexes compared to the lipoplexes, in A549 cells in vitro.CONCLUSIONS f-MWNTs-cationic liposome hybrid designed in this study can serve as a potential vehicle for the co-delivery of siRNA and cytotoxic drugs to cancer cells in vitro.",

keywords = "Ammonium Compounds, Antineoplastic Agents, Apoptosis, Cations, Cell Line, Tumor, Chemistry, Pharmaceutical, Doxorubicin, Drug Delivery Systems, Humans, Liposomes, Nanotubes, Carbon, RNA, Small Interfering, Journal Article, Research Support, Non-U.S. Gov't",

author = "Sara Pereira and Jin Lee and Noelia Rubio and Hassan, {Hatem A F M} and Suffian, {Izzat Bin Mohamed} and Wang, {Julie T-W} and Rebecca Klippstein and Bel{\'e}n Ballesteros and Al-Jamal, {Wafa' T} and Al-Jamal, {Khuloud T}",

year = "2015",

month = oct,

doi = "10.1007/s11095-015-1707-1",

language = "English",

volume = "32",

pages = "3293--308",

journal = "Pharmaceutical Research",

issn = "0724-8741",

publisher = "Springer",

number = "10",

}

TY - JOUR

T1 - Cationic Liposome- Multi-Walled Carbon Nanotubes Hybrids for Dual siPLK1 and Doxorubicin Delivery In Vitro

AU - Pereira, Sara

AU - Lee, Jin

AU - Rubio, Noelia

AU - Hassan, Hatem A F M

AU - Suffian, Izzat Bin Mohamed

AU - Wang, Julie T-W

AU - Klippstein, Rebecca

AU - Ballesteros, Belén

AU - Al-Jamal, Wafa' T

AU - Al-Jamal, Khuloud T

PY - 2015/10

Y1 - 2015/10

N2 - PURPOSETo formulate f-MWNTs-cationic liposome hybrids for the simultaneous delivery of siPLK1 and doxorubicin to cancer cells.METHOD f-MWNTs-cationic liposome hybrids were prepared by the thin film hydration method where the lipid film was hydrated with 100 μg/ml or 1 mg/ml of ox-MWNTs-NH3 (+) or MWNTs-NH3 (+) in 5% dextrose. siRNA complexation and protection ability was determined by agarose gel electrophoresis. f-MWNTs and liposome interaction was evaluated using Nile Red (NR) fluorescence spectroscopy. Cellular uptake in A549 cells was assessed by flow cytometry. Silencing of target proteins was determined by Luciferase and MTT assays. Sub-G1 analysis was performed to evaluate apoptosis following co-delivery of siPLK1 and Doxorubicin (Dox).RESULTS Zeta potential and siRNA complexation profile obtained for all hybrids were comparable to those achieved with cationic liposomes. ox-MWNTs-NH3 (+) showed greater extent of interaction with cationic liposomes compared to MWNTs-NH3 (+). ox-MWNTs-NH3 (+) was able to protect siRNA from nuclease-mediated degradation. Enhanced cellular uptake of both the carrier and loaded siRNA in A549 cell, were observed for this hybrid compared to the liposomal carrier. A synergistic pro-apoptotic effect was obtained when siPLK1 silencing was combined with doxorubicin treatment for the hybrid:siRNA complexes compared to the lipoplexes, in A549 cells in vitro.CONCLUSIONS f-MWNTs-cationic liposome hybrid designed in this study can serve as a potential vehicle for the co-delivery of siRNA and cytotoxic drugs to cancer cells in vitro.

AB - PURPOSETo formulate f-MWNTs-cationic liposome hybrids for the simultaneous delivery of siPLK1 and doxorubicin to cancer cells.METHOD f-MWNTs-cationic liposome hybrids were prepared by the thin film hydration method where the lipid film was hydrated with 100 μg/ml or 1 mg/ml of ox-MWNTs-NH3 (+) or MWNTs-NH3 (+) in 5% dextrose. siRNA complexation and protection ability was determined by agarose gel electrophoresis. f-MWNTs and liposome interaction was evaluated using Nile Red (NR) fluorescence spectroscopy. Cellular uptake in A549 cells was assessed by flow cytometry. Silencing of target proteins was determined by Luciferase and MTT assays. Sub-G1 analysis was performed to evaluate apoptosis following co-delivery of siPLK1 and Doxorubicin (Dox).RESULTS Zeta potential and siRNA complexation profile obtained for all hybrids were comparable to those achieved with cationic liposomes. ox-MWNTs-NH3 (+) showed greater extent of interaction with cationic liposomes compared to MWNTs-NH3 (+). ox-MWNTs-NH3 (+) was able to protect siRNA from nuclease-mediated degradation. Enhanced cellular uptake of both the carrier and loaded siRNA in A549 cell, were observed for this hybrid compared to the liposomal carrier. A synergistic pro-apoptotic effect was obtained when siPLK1 silencing was combined with doxorubicin treatment for the hybrid:siRNA complexes compared to the lipoplexes, in A549 cells in vitro.CONCLUSIONS f-MWNTs-cationic liposome hybrid designed in this study can serve as a potential vehicle for the co-delivery of siRNA and cytotoxic drugs to cancer cells in vitro.

KW - Ammonium Compounds

KW - Antineoplastic Agents

KW - Apoptosis

KW - Cations

KW - Cell Line, Tumor

KW - Chemistry, Pharmaceutical

KW - Doxorubicin

KW - Drug Delivery Systems

KW - Humans

KW - Liposomes

KW - Nanotubes, Carbon

KW - RNA, Small Interfering

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/s11095-015-1707-1

DO - 10.1007/s11095-015-1707-1

M3 - Article

C2 - 26085038

SN - 0724-8741

VL - 32

SP - 3293

EP - 3308

JO - Pharmaceutical Research

JF - Pharmaceutical Research

IS - 10

ER -

Cationic Liposome- Multi-Walled Carbon Nanotubes Hybrids for Dual siPLK1 and Doxorubicin Delivery In Vitro

Abstract

Keywords

UN SDGs

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