Cell-to-cell variations in subcellular localisation of V2R are independent of its expression level

Eline Koers, Bradley Morgan, Franziska M. Heydenreich, Bianca Plouffe, Iain Styles, Dmitry Veprintsev*

*Corresponding author for this work

Research output: Other contribution

Abstract

G protein coupled receptors (GPCRs) translate the actions of hormones into intracellular signalling events. Mutations in GPCRs can prevent their correct expression and trafficking to the cell surface and cause disease. We use single cell measurements in HEK293 cells to show that the balance between endoplasmic reticulum (ER) and cell surface localisation of the Vasopressin 2 receptor (V2R) varies significantly from cell to cell. We find that mutations in the V2R affect the proportion of cells able to send this GPCR to the cell surface but do not prevent all cells in the population from correctly trafficking the mutant receptors. These findings reveal that the ability of cells to correctly traffic V2R to the cell surface depends not only on the expressed V2R mutant but also on the individual cell environment.

Original languageEnglish
TypePreprint
Media of outputOnline preprint archive
PublisherBioRxiv
DOIs
Publication statusPublished - 25 Feb 2022

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