Characterisation of a beta-tubulin gene from the liver fluke, Fasciola hepatica.

Elizabeth Hoey, Mark Robinson, Ian Fairweather, J.P. Dalton, S. McGonigle, Alan Trudgett

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

This study represents the first ß-tubulin sequence from a trematode parasite, namely, the liver fluke, Fasciola hepatica. PCR of genomic DNA showed that at least one ß-tubulin gene from F. hepatica contains no introns. A number of amino acids in the primary sequence of fluke tubulin are different from those described previously in various nematode species and the cestode, Echinococcus multilocularis. ß-Tubulin is an important target for benzimidazole anthelmintics, although (with the exception of triclabendazole) they show limited activity against F. hepatica. The amino acid differences in fluke ß-tubulin are discussed in relation to the selective toxicity of benzimidazoles against helminths and the mechanism of drug resistance.
Original languageEnglish
Pages (from-to)1264-1268
Number of pages5
JournalInternational Journal for Parasitology
Volume31(11)
Issue number11
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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