Characterising the TP53-deleted subgroup of chronic lymphocytic leukemia: an analysis of additional cytogenetic abnormalities detected by interphase fluorescence in situ hybridisation and array-based comparative genomic hybridisation.

H.C. Rudenko, M. Else, C. Dearden, V. Brito-Babapulle, C. Jones, T. Dexter, K. Fenwick, A. Mackay, A. Ashworth, E. Matutes, D. Gonzalez, D. Catovsky, G.J. Morgan

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Deletion of the TP53 gene on chromosome 17p13.1 is the prognostic factor associated with the shortest survival in CLL. We used array-based comparative genomic hybridisation (arrayCGH) to identify additional DNA copy number changes in peripheral blood samples from 74 LRF CLL4 trial patients, 37 with >or=5% and 37 without TP53-deleted cells. ArrayCGH reliably detected deletions on 17p, including the TP53 locus, in cases with >or=50%TP53-deleted cells detected by fluorescence in situ hybridisation, plus seven additional cases with deleted regions on 17p excluding TP53. Losses on chromosomal regions 18p and/or 20p were found exclusively in cases with >or=5%TP53-deleted cells (por=5%TP53-deleted cases (p=0.02). In particular, amplification of 2p and deletion of 6q were both more frequent. Cases with >20%TP53-deleted cells had the worst prognosis in the LRF CLL4 trial.
Original languageEnglish
Pages (from-to)1879-1886
Number of pages8
JournalLeuk Lymphoma
Volume49
Issue number10
DOIs
Publication statusPublished - Oct 2008

Keywords

  • Aged
  • Chromosome Aberrations
  • Chromosome Deletion
  • Chromosomes, Human, Pair 17
  • Chromosomes, Human, Pair 18
  • Chromosomes, Human, Pair 20
  • Clinical Trials as Topic
  • Comparative Genomic Hybridization
  • Female
  • Humans
  • In Situ Hybridization, Fluorescence
  • Leukemia, Lymphocytic, Chronic, B-Cell
  • Male
  • Prognosis
  • Tumor Suppressor Protein p53

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