Characterization of melanoidins from a glucose-glycine model system

R.C. Borrelli; V. Fogliano; S.M. Monti; Jennifer Ames

doi:10.1007/s00217-002-0531-0

Characterization of melanoidins from a glucose-glycine model system

R.C. Borrelli, V. Fogliano, S.M. Monti, Jennifer Ames

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

The properties of melanoidins prepared from glucose and glycine (GG) were investigated by a three step purification protocol consisting of dialysis, gel filtration at high ionic strength and ion metal affinity chromatography. The high molecular weight fraction obtained in the GG system is responsible for 80% of the total brown colour and its antioxidative ability was about 1/4 of that of Trolox measured by the inhibition of linoleic acid oxidation. GG melanoidins have good affinity towards Cu (II) (32% bound to the resin) while it is much lower towards Pb (II) (10%) and Fe (II) (5%). Capillary zone electrophoresis analysis suggests that GG melanoidins are positively charged, although no signal was observed analysing melanoidins by matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry (MALDI-TOF/MS).

Original language	English
Pages (from-to)	210-215
Number of pages	6
Journal	European Food Research and Technology
Volume	215(3)
Issue number	3
DOIs	https://doi.org/10.1007/s00217-002-0531-0
Publication status	Published - Sept 2002

ASJC Scopus subject areas

Food Science

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abstract = "The properties of melanoidins prepared from glucose and glycine (GG) were investigated by a three step purification protocol consisting of dialysis, gel filtration at high ionic strength and ion metal affinity chromatography. The high molecular weight fraction obtained in the GG system is responsible for 80% of the total brown colour and its antioxidative ability was about 1/4 of that of Trolox measured by the inhibition of linoleic acid oxidation. GG melanoidins have good affinity towards Cu (II) (32% bound to the resin) while it is much lower towards Pb (II) (10%) and Fe (II) (5%). Capillary zone electrophoresis analysis suggests that GG melanoidins are positively charged, although no signal was observed analysing melanoidins by matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry (MALDI-TOF/MS).",

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AU - Borrelli, R.C.

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AU - Monti, S.M.

AU - Ames, Jennifer

PY - 2002/9

Y1 - 2002/9

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Characterization of melanoidins from a glucose-glycine model system

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