Characterization of polymorphic microsatellites for the rough periwinkle gastropod, Littorina saxatilis (Olivi, 1792) and their cross-amplification in four congeners.

C.E. McInerney; Louise Allcock; Mark Johnson; Paulo Prodöhl

doi:10.1007/s10592-009-9875-3

Characterization of polymorphic microsatellites for the rough periwinkle gastropod, Littorina saxatilis (Olivi, 1792) and their cross-amplification in four congeners.

C.E. McInerney, Louise Allcock, Mark Johnson, Paulo Prodöhl

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

253 Downloads (Pure)

Abstract

Eight new microsatellite loci were characterized for Littorina saxatilis (Olivi, 1792) and tested for their cross-hybridization in congeners. All loci were polymorphic in Irish and Celtic Sea samples, with an average number of alleles per locus of 15 (range, 6–31). Observed and expected locus heterozygosities ranged from 26 to 85% and from 53 to 92%, respectively. Three loci showed excess homozygosity and significant departures from Hardy–Weinberg expectations in one sample, possibly due to null alleles, population structuring or inbreeding. No linkage disequilibrium was detected among loci within samples. A high degree of cross-hybridization was observed in closely related congeners and most loci were polymorphic. These markers will be useful for investigating population genetic diversity and connectivity in coastal populations, especially for marine reserve design.

Original language	English
Pages (from-to)	1989-1992
Number of pages	4
Journal	Conservation Genetics
Volume	10
Issue number	6
Early online date	10 Mar 2009
DOIs	https://doi.org/10.1007/s10592-009-9875-3
Publication status	Published - Dec 2009

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/s10592-009-9875-3Licence: Unspecified

Cite this

@article{b83b2454bf3d49a88ea965587af1da16,

title = "Characterization of polymorphic microsatellites for the rough periwinkle gastropod, Littorina saxatilis (Olivi, 1792) and their cross-amplification in four congeners.",

abstract = "Eight new microsatellite loci were characterized for Littorina saxatilis (Olivi, 1792) and tested for their cross-hybridization in congeners. All loci were polymorphic in Irish and Celtic Sea samples, with an average number of alleles per locus of 15 (range, 6–31). Observed and expected locus heterozygosities ranged from 26 to 85% and from 53 to 92%, respectively. Three loci showed excess homozygosity and significant departures from Hardy–Weinberg expectations in one sample, possibly due to null alleles, population structuring or inbreeding. No linkage disequilibrium was detected among loci within samples. A high degree of cross-hybridization was observed in closely related congeners and most loci were polymorphic. These markers will be useful for investigating population genetic diversity and connectivity in coastal populations, especially for marine reserve design.",

author = "C.E. McInerney and Louise Allcock and Mark Johnson and Paulo Prod{\"o}hl",

year = "2009",

month = dec,

doi = "10.1007/s10592-009-9875-3",

language = "English",

volume = "10",

pages = "1989--1992",

journal = "Conservation Genetics",

issn = "1572-9737",

publisher = "Springer Science and Business Media B.V.",

number = "6",

}

TY - JOUR

T1 - Characterization of polymorphic microsatellites for the rough periwinkle gastropod, Littorina saxatilis (Olivi, 1792) and their cross-amplification in four congeners.

AU - McInerney, C.E.

AU - Allcock, Louise

AU - Johnson, Mark

AU - Prodöhl, Paulo

PY - 2009/12

Y1 - 2009/12

N2 - Eight new microsatellite loci were characterized for Littorina saxatilis (Olivi, 1792) and tested for their cross-hybridization in congeners. All loci were polymorphic in Irish and Celtic Sea samples, with an average number of alleles per locus of 15 (range, 6–31). Observed and expected locus heterozygosities ranged from 26 to 85% and from 53 to 92%, respectively. Three loci showed excess homozygosity and significant departures from Hardy–Weinberg expectations in one sample, possibly due to null alleles, population structuring or inbreeding. No linkage disequilibrium was detected among loci within samples. A high degree of cross-hybridization was observed in closely related congeners and most loci were polymorphic. These markers will be useful for investigating population genetic diversity and connectivity in coastal populations, especially for marine reserve design.

AB - Eight new microsatellite loci were characterized for Littorina saxatilis (Olivi, 1792) and tested for their cross-hybridization in congeners. All loci were polymorphic in Irish and Celtic Sea samples, with an average number of alleles per locus of 15 (range, 6–31). Observed and expected locus heterozygosities ranged from 26 to 85% and from 53 to 92%, respectively. Three loci showed excess homozygosity and significant departures from Hardy–Weinberg expectations in one sample, possibly due to null alleles, population structuring or inbreeding. No linkage disequilibrium was detected among loci within samples. A high degree of cross-hybridization was observed in closely related congeners and most loci were polymorphic. These markers will be useful for investigating population genetic diversity and connectivity in coastal populations, especially for marine reserve design.

U2 - 10.1007/s10592-009-9875-3

DO - 10.1007/s10592-009-9875-3

M3 - Article

SN - 1572-9737

VL - 10

SP - 1989

EP - 1992

JO - Conservation Genetics

JF - Conservation Genetics

IS - 6

ER -

Characterization of polymorphic microsatellites for the rough periwinkle gastropod, Littorina saxatilis (Olivi, 1792) and their cross-amplification in four congeners.

Abstract

ASJC Scopus subject areas

UN SDGs

Access to Document

Fingerprint

Cite this